Abstract
Membrane–nanoparticle interactions are important in determining the effects
of manufactured nanomaterials on cell physiology and pathology. Here, silica,
titanium, zinc, and magnesium oxide nanoparticles are screened against
human hERG (Kv11.1) voltage-gated potassium channels under a whole-cell
voltage clamp. 10 μg mL−1 ZnO uniquely increases the amplitude of the
steady-state current, decreases the rate of hERG current inactivation during
steady-state depolarization, accelerates channel deactivation during resurgent
tail currents, and shows no significant alteration of current activation rate or
voltage dependence. In contrast, ZnCl2 causes increased current suppression
with increasing concentration and fails to replicate the nanoparticle effect
on decreasing inactivation. The results show a novel class of nanoparticle–
biomembrane interaction involving channel gating rather than channel block,
and have implications for the use of nanoparticles in biomedicine, drug
delivery applications, and nanotoxicology.
of manufactured nanomaterials on cell physiology and pathology. Here, silica,
titanium, zinc, and magnesium oxide nanoparticles are screened against
human hERG (Kv11.1) voltage-gated potassium channels under a whole-cell
voltage clamp. 10 μg mL−1 ZnO uniquely increases the amplitude of the
steady-state current, decreases the rate of hERG current inactivation during
steady-state depolarization, accelerates channel deactivation during resurgent
tail currents, and shows no significant alteration of current activation rate or
voltage dependence. In contrast, ZnCl2 causes increased current suppression
with increasing concentration and fails to replicate the nanoparticle effect
on decreasing inactivation. The results show a novel class of nanoparticle–
biomembrane interaction involving channel gating rather than channel block,
and have implications for the use of nanoparticles in biomedicine, drug
delivery applications, and nanotoxicology.
Original language | English |
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Article number | 1703403 |
Journal | Small |
Volume | 14 |
Issue number | 15 |
Early online date | 26 Feb 2018 |
DOIs | |
Publication status | Published - 12 Apr 2018 |
Keywords
- channel gating
- hERG potassium channel
- lipid bilayer
- nanoparticles
- zinc oxide
ASJC Scopus subject areas
- General Materials Science
- General Chemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
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Euan R. Brown
- School of Engineering & Physical Sciences - Associate Professor
- School of Engineering & Physical Sciences, Institute of Biological Chemistry, Biophysics and Bioengineering - Associate Professor
Person: Academic (Research & Teaching)