TY - JOUR
T1 - Valorization of chicken slaughterhouse by-products: Production and properties of chicken trachea hydrolysates using commercial proteases
AU - Pramualkijja, Teeda
AU - Pirak, Tantawan
AU - Euston, Stephen R.
N1 - Funding Information:
This work was supported by the Thailand Research Fund:?Research and Researchers for Industries for Ph.D. (RRI-Ph.D.)?[PHD59I0014]. The authors gratefully acknowledge Research and Researchers for Industries?RRI (PHD59I0014) for funding this research, CPF Food Research and Development Center for financial and ingredient supports, Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Thailand, and School of Engineering and Physical Sciences, Heriot-Watt University for instrumental support of this work. The authors declare that there is no conflict of interest.
Funding Information:
The authors gratefully acknowledge Research and Researchers for Industries – RRI (PHD59I0014) for funding this research, CPF Food Research and Development Center for financial and ingredient supports, Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Thailand, and School of Engineering and Physical Sciences, Heriot-Watt University for instrumental support of this work. The authors declare that there is no conflict of interest.
Publisher Copyright:
© 2021 Teeda Pramualkijja, Tantawan Pirak and Stephen R. Euston. Published with license by Taylor & Francis Group, LLC. © 2021, Published with license by Taylor & Francis Group, LLC. © 2021 Teeda Pramualkijja, Tantawan Pirak and Stephen R. Euston.
PY - 2021
Y1 - 2021
N2 - Collagen is an abundant structural protein found in many organs of animals, for example skin, bones, and connective tissues. Chicken trachea is a collagen-rich fraction of chicken offal that is converted to a solid waste stream during poultry processing. Collagen hydrolysates and peptides have unique biological properties, which is potentially converted from chicken trachea and can be used as a useful functional ingredient. Fresh chicken trachea was sterilized by autoclaving at 121°C for 30 min and dried using freeze drier to obtain chicken trachea flakes (CTF). CTF had 68.56% protein with the 50% solubility at pH 6–10. The enzymatic hydrolysis of chicken trachea was performed using Alcalase®, Flavourzyme®, Protamex®, and Papain under the optimal condition of each enzyme. An improvement in solubility was observed. Chicken trachea hydrolysates (CTH) with the highest solubility were detected in samples hydrolyzed with Alcalase®, follow by Protamex®, Papain, and Flavourzyme®, respectively. Within an hour of hydrolysis, the CTH obtained from Alcalase® hydrolysis had the highest total amino acid contents. The top three amino acids found in this hydrolyzate powder were Glutamic acid (Glu), Glycine (Gly), and Aspartic acid (Asp). Moreover, this sample exhibited the greatest antioxidant and bioactive properties as shown by the highest antioxidant capacity from DPPH and FRAP assay (4.42 TEAC µMol/mg CTH and 22.48 TEAC µMol/mg CTH, respectively) and the lowest IC50 of ACE I inhibitor (0.41 mg/mL). These results suggested that Alcalase® hydrolysis provided the chicken trachea collagen hydrolyzate with unique properties that could be used in various food systems.
AB - Collagen is an abundant structural protein found in many organs of animals, for example skin, bones, and connective tissues. Chicken trachea is a collagen-rich fraction of chicken offal that is converted to a solid waste stream during poultry processing. Collagen hydrolysates and peptides have unique biological properties, which is potentially converted from chicken trachea and can be used as a useful functional ingredient. Fresh chicken trachea was sterilized by autoclaving at 121°C for 30 min and dried using freeze drier to obtain chicken trachea flakes (CTF). CTF had 68.56% protein with the 50% solubility at pH 6–10. The enzymatic hydrolysis of chicken trachea was performed using Alcalase®, Flavourzyme®, Protamex®, and Papain under the optimal condition of each enzyme. An improvement in solubility was observed. Chicken trachea hydrolysates (CTH) with the highest solubility were detected in samples hydrolyzed with Alcalase®, follow by Protamex®, Papain, and Flavourzyme®, respectively. Within an hour of hydrolysis, the CTH obtained from Alcalase® hydrolysis had the highest total amino acid contents. The top three amino acids found in this hydrolyzate powder were Glutamic acid (Glu), Glycine (Gly), and Aspartic acid (Asp). Moreover, this sample exhibited the greatest antioxidant and bioactive properties as shown by the highest antioxidant capacity from DPPH and FRAP assay (4.42 TEAC µMol/mg CTH and 22.48 TEAC µMol/mg CTH, respectively) and the lowest IC50 of ACE I inhibitor (0.41 mg/mL). These results suggested that Alcalase® hydrolysis provided the chicken trachea collagen hydrolyzate with unique properties that could be used in various food systems.
KW - By-products
KW - Chicken trachea hydrolysates
KW - Commercial proteases
KW - Enzymatic hydrolysis
KW - Valorization
UR - http://www.scopus.com/inward/record.url?scp=85117169330&partnerID=8YFLogxK
U2 - 10.1080/10942912.2021.1986522
DO - 10.1080/10942912.2021.1986522
M3 - Article
SN - 1094-2912
VL - 24
SP - 1642
EP - 1657
JO - International Journal of Food Properties
JF - International Journal of Food Properties
IS - 1
ER -