TY - JOUR
T1 - Validation of determinate (dt) gene-based DNA marker in inter-specific hybrid sesame and in-silico analysis of the predicted dt protein structures
AU - Dutta, Debabrata
AU - Banerjee, Saptadipa
AU - Pal, Manisha
AU - Gangopadhyay, Gaurab
PY - 2022/1/31
Y1 - 2022/1/31
N2 - Determinacy is a desirable trait in sesame, an important oilseed crop. We have developed an inter-specific hybrid between basally branched indeterminate cultivated Sesamum indicum genotype and wild S. prostratum with no branching yet synchronous pods on the shoot. The hybrid and a few exotic sesame germplasms were successfully screened with a determinacy (dt) gene-based DNA marker. In-silico translation of the partial coding sequences of the dt gene from the two contrasting parent genotypes revealed an SNP (V159A) in S. prostratum. The predicted cytoplasmic dt protein showed a high resemblance with flowering protein centroradialis.
AB - Determinacy is a desirable trait in sesame, an important oilseed crop. We have developed an inter-specific hybrid between basally branched indeterminate cultivated Sesamum indicum genotype and wild S. prostratum with no branching yet synchronous pods on the shoot. The hybrid and a few exotic sesame germplasms were successfully screened with a determinacy (dt) gene-based DNA marker. In-silico translation of the partial coding sequences of the dt gene from the two contrasting parent genotypes revealed an SNP (V159A) in S. prostratum. The predicted cytoplasmic dt protein showed a high resemblance with flowering protein centroradialis.
U2 - 10.1007/s12298-022-01135-1
DO - 10.1007/s12298-022-01135-1
M3 - Article
SN - 0971-5894
VL - 28
SP - 139
EP - 152
JO - Physiology and Molecular Biology of Plants
JF - Physiology and Molecular Biology of Plants
IS - 1
ER -