Validation of a blood plasma separation system by biomarker detection

Maïwenn Kersaudy-Kerhoas, Deirdre M. Kavanagh, Resham S. Dhariwal, Colin J. Campbell, M. P Y Desmulliez

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)
224 Downloads (Pure)

Abstract

A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity efficiency of 100% is obtained on blood with entrance hematocrit level up to 30% with a flow rate of 2 mL h-1. Flow cytometry was performed on the extracted plasma to evaluate the separation efficiency and to assess cell damage. A core target of this study was the detection of cell-free DNA from the on-chip extracted plasma. To this effect, PCR was successfully carried out off-chip on the cell-free DNA present in the plasma extracted on-chip. A house-keeping gene sequence (GAPDH) was amplified without the need for a purification after the separation, thereby showing the high quality of the plasma sample. The resulting data suggests that the system can be used as a preliminary module of a total analysis system for cell-free DNA detection in human plasma. © 2010 The Royal Society of Chemistry.

Original languageEnglish
Pages (from-to)1587-1595
Number of pages9
JournalLab on a Chip
Volume10
Issue number12
DOIs
Publication statusPublished - 2010

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