Use of dimensionless residence time to study variations in breakthrough behaviour in expanded beds formed from varied particle size distributions

P. J. Gardner, Nicholas Willoughby, R. Hjorth, K. Lacki, Nigel J. Titchener-Hooker

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This work demonstrates an experimental method for studying breakthrough behaviour in expanded beds. The behaviour of beds made with differently sized particles were studied at varying flowrates. The use of a dimensionless residence time measurement allowed a more valid comparison of breakthrough characteristics in expanded bed operation by compensating for the changes in bed volume that occur during expansion. We demonstrate that bed breakthrough behaviour can be compared directly even when the beds contain different-sized particles and hence have different expanded volumes. By utilising this concept we demonstrate that, in the case of the Alcohol Dehydrogenase (ADH) / STREAMLINE Phenyl system used here, there was little or no variation in ADH breakthrough behaviour between beds of differently sized particles operating at flowrates above 100 cm/h. This suggests that the higher specific surface area and hence binding capacity of smaller particles is negated in this case due to mass transfer limitations and the increase in system void volume even at normal operating flowrates of 200–300 cm/h.
Original languageEnglish
Pages (from-to)347-353
Number of pages7
JournalBiotechnology and Bioengineering
Volume87
Issue number3
DOIs
Publication statusPublished - 5 Aug 2004

Keywords

  • Breakthrough behaviour
  • Expanded bed adsorption
  • Residence time
  • Segregated beds

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Fingerprint Dive into the research topics of 'Use of dimensionless residence time to study variations in breakthrough behaviour in expanded beds formed from varied particle size distributions'. Together they form a unique fingerprint.

Cite this