Uptake, depuration, and radiation dose estimation in zebrafish exposed to radionuclides via aqueous or dietary routes

Helena C. Reinardy, Jean-Louis Teyssie, Ross A. Jeffree, David Copplestone, Theodore B. Henry, Awadhesh N. Jha

    Research output: Contribution to journalArticle

    Abstract

    Understanding uptake and deputation of radionuclides in organisms is necessary to relate exposure to radiation dose and ultimately to biological effects. We investigated uptake and depuration of a mixture of radionuclides to link bioaccumulation with radiation dose in zebrafish, Danio rerio. Adult zebrafish were exposed to radionuclides ((54)Mn, (60)Co, (65)Zn, (75)Se, (109)Cd, (110m)Ag, (134)Cs and (241)Am) at tracer levels ((65)Zn>(109)Cd>(110m)Ag>(54)Mn>(60)Co>(241)Am>(134)Cs (range: 175-8 Bq g(1)). After dietary exposure the order of radionuclide activity concentration in tissues (Bq g(-1)) was: (65)Zn>(60)Co>(75)Se>(109)Cd>(110m)Ag>(241)Am>(54)Mn>(134)Cs (range: 91-1 Bq g(-1)). Aqueous exposure resulted in higher whole body activity concentrations for all radionuclides except (60)Co. Route of exposure did not appear to influence activity concentrations in gonads, except for (54)Mn, (65)Zn, and (75)Se, which had higher activity concentrations in gonads following aqueous exposure. Highest gonad activity concentrations (Bq g(-1)) were for (75)Se (211), (109)Cd (142), and (65)Zn (117), and highest dose rates (mu Gy h(-1)) were from (241)Am (aqueous, 1050; diet 242). This study links radionuclide bioaccumulation data obtained in laboratory experiments with radiation dose determined by application of a dosimetry modelling tool, an approach that will enable better linkages to be made between exposure, dose, and effects of radionuclides in organisms. (C) 2011 Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)3771-3779
    Number of pages9
    JournalScience of the Total Environment
    Volume409
    Issue number19
    DOIs
    Publication statusPublished - 1 Sep 2011

    Keywords

    • COMET ASSAY
    • TISSUE
    • FISH
    • Radiation dosimetry
    • HEAVY-METALS
    • Zebrafish
    • DOGFISH SCYLIORHINUS-CANICULA
    • MODEL
    • Bioaccumulation
    • MARINE MUSSEL
    • BIOACCUMULATION
    • ERICA Tool
    • Radionuclides
    • MYTILUS-EDULIS
    • ERICA INTEGRATED APPROACH

    Cite this

    Reinardy, Helena C. ; Teyssie, Jean-Louis ; Jeffree, Ross A. ; Copplestone, David ; Henry, Theodore B. ; Jha, Awadhesh N. / Uptake, depuration, and radiation dose estimation in zebrafish exposed to radionuclides via aqueous or dietary routes. In: Science of the Total Environment. 2011 ; Vol. 409, No. 19. pp. 3771-3779.
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    Uptake, depuration, and radiation dose estimation in zebrafish exposed to radionuclides via aqueous or dietary routes. / Reinardy, Helena C.; Teyssie, Jean-Louis; Jeffree, Ross A.; Copplestone, David; Henry, Theodore B.; Jha, Awadhesh N.

    In: Science of the Total Environment, Vol. 409, No. 19, 01.09.2011, p. 3771-3779.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Uptake, depuration, and radiation dose estimation in zebrafish exposed to radionuclides via aqueous or dietary routes

    AU - Reinardy, Helena C.

    AU - Teyssie, Jean-Louis

    AU - Jeffree, Ross A.

    AU - Copplestone, David

    AU - Henry, Theodore B.

    AU - Jha, Awadhesh N.

    PY - 2011/9/1

    Y1 - 2011/9/1

    N2 - Understanding uptake and deputation of radionuclides in organisms is necessary to relate exposure to radiation dose and ultimately to biological effects. We investigated uptake and depuration of a mixture of radionuclides to link bioaccumulation with radiation dose in zebrafish, Danio rerio. Adult zebrafish were exposed to radionuclides ((54)Mn, (60)Co, (65)Zn, (75)Se, (109)Cd, (110m)Ag, (134)Cs and (241)Am) at tracer levels ((65)Zn>(109)Cd>(110m)Ag>(54)Mn>(60)Co>(241)Am>(134)Cs (range: 175-8 Bq g(1)). After dietary exposure the order of radionuclide activity concentration in tissues (Bq g(-1)) was: (65)Zn>(60)Co>(75)Se>(109)Cd>(110m)Ag>(241)Am>(54)Mn>(134)Cs (range: 91-1 Bq g(-1)). Aqueous exposure resulted in higher whole body activity concentrations for all radionuclides except (60)Co. Route of exposure did not appear to influence activity concentrations in gonads, except for (54)Mn, (65)Zn, and (75)Se, which had higher activity concentrations in gonads following aqueous exposure. Highest gonad activity concentrations (Bq g(-1)) were for (75)Se (211), (109)Cd (142), and (65)Zn (117), and highest dose rates (mu Gy h(-1)) were from (241)Am (aqueous, 1050; diet 242). This study links radionuclide bioaccumulation data obtained in laboratory experiments with radiation dose determined by application of a dosimetry modelling tool, an approach that will enable better linkages to be made between exposure, dose, and effects of radionuclides in organisms. (C) 2011 Elsevier B.V. All rights reserved.

    AB - Understanding uptake and deputation of radionuclides in organisms is necessary to relate exposure to radiation dose and ultimately to biological effects. We investigated uptake and depuration of a mixture of radionuclides to link bioaccumulation with radiation dose in zebrafish, Danio rerio. Adult zebrafish were exposed to radionuclides ((54)Mn, (60)Co, (65)Zn, (75)Se, (109)Cd, (110m)Ag, (134)Cs and (241)Am) at tracer levels ((65)Zn>(109)Cd>(110m)Ag>(54)Mn>(60)Co>(241)Am>(134)Cs (range: 175-8 Bq g(1)). After dietary exposure the order of radionuclide activity concentration in tissues (Bq g(-1)) was: (65)Zn>(60)Co>(75)Se>(109)Cd>(110m)Ag>(241)Am>(54)Mn>(134)Cs (range: 91-1 Bq g(-1)). Aqueous exposure resulted in higher whole body activity concentrations for all radionuclides except (60)Co. Route of exposure did not appear to influence activity concentrations in gonads, except for (54)Mn, (65)Zn, and (75)Se, which had higher activity concentrations in gonads following aqueous exposure. Highest gonad activity concentrations (Bq g(-1)) were for (75)Se (211), (109)Cd (142), and (65)Zn (117), and highest dose rates (mu Gy h(-1)) were from (241)Am (aqueous, 1050; diet 242). This study links radionuclide bioaccumulation data obtained in laboratory experiments with radiation dose determined by application of a dosimetry modelling tool, an approach that will enable better linkages to be made between exposure, dose, and effects of radionuclides in organisms. (C) 2011 Elsevier B.V. All rights reserved.

    KW - COMET ASSAY

    KW - TISSUE

    KW - FISH

    KW - Radiation dosimetry

    KW - HEAVY-METALS

    KW - Zebrafish

    KW - DOGFISH SCYLIORHINUS-CANICULA

    KW - MODEL

    KW - Bioaccumulation

    KW - MARINE MUSSEL

    KW - BIOACCUMULATION

    KW - ERICA Tool

    KW - Radionuclides

    KW - MYTILUS-EDULIS

    KW - ERICA INTEGRATED APPROACH

    U2 - 10.1016/j.scitotenv.2011.06.057

    DO - 10.1016/j.scitotenv.2011.06.057

    M3 - Article

    C2 - 21782211

    VL - 409

    SP - 3771

    EP - 3779

    JO - Science of the Total Environment

    JF - Science of the Total Environment

    SN - 0048-9697

    IS - 19

    ER -