Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging

Harrisson D. A. Santos; Irene Zabala Gutiérrez; Yingli Shen; José Lifante; Erving Ximendes; Marco Laurenti; Diego Méndez-González; Sonia Melle; Oscar G. Calderón; Enrique López Cabarcos; Nuria Fernández; Irene Chaves-Coira; Daniel Lucena-Agell; Luis Monge; Mark D. Mackenzie; José Marqués-Hueso; Callum M. S. Jones; Carlos Jacinto; Blanca del Rosal; Ajoy K. Kar; Jorge Rubio-Retama; Daniel Jaque

doi:10.1038/s41467-020-16333-2

Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging

Harrisson D. A. Santos, Irene Zabala Gutiérrez, Yingli Shen, José Lifante, Erving Ximendes, Marco Laurenti, Diego Méndez-González, Sonia Melle, Oscar G. Calderón, Enrique López Cabarcos, Nuria Fernández, Irene Chaves-Coira, Daniel Lucena-Agell, Luis Monge, Mark D. Mackenzie, José Marqués-Hueso, Callum M. S. Jones, Carlos Jacinto, Blanca del Rosal, Ajoy K. KarJorge Rubio-Retama, Daniel Jaque

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Abstract

Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm-2) and doses (<0.5 mg kg-1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.

Original language	English
Article number	2933
Journal	Nature Communications
Volume	11
Issue number	1
DOIs	https://doi.org/10.1038/s41467-020-16333-2
Publication status	Published - 10 Jun 2020

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

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Santos, H. D. A., Zabala Gutiérrez, I., Shen, Y., Lifante, J., Ximendes, E., Laurenti, M., Méndez-González, D., Melle, S., Calderón, O. G., López Cabarcos, E., Fernández, N., Chaves-Coira, I., Lucena-Agell, D., Monge, L., Mackenzie, M. D., Marqués-Hueso, J., Jones, C. M. S., Jacinto, C., del Rosal, B., ... Jaque, D. (2020). Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging. Nature Communications, 11(1), Article 2933. https://doi.org/10.1038/s41467-020-16333-2

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title = "Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging",

abstract = "Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm-2) and doses (<0.5 mg kg-1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.",

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year = "2020",

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Santos, HDA, Zabala Gutiérrez, I, Shen, Y, Lifante, J, Ximendes, E, Laurenti, M, Méndez-González, D, Melle, S, Calderón, OG, López Cabarcos, E, Fernández, N, Chaves-Coira, I, Lucena-Agell, D, Monge, L, Mackenzie, MD, Marqués-Hueso, J, Jones, CMS, Jacinto, C, del Rosal, B, Kar, AK, Rubio-Retama, J & Jaque, D 2020, 'Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging', Nature Communications, vol. 11, no. 1, 2933. https://doi.org/10.1038/s41467-020-16333-2

TY - JOUR

T1 - Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging

AU - Santos, Harrisson D. A.

AU - Zabala Gutiérrez, Irene

AU - Shen, Yingli

AU - Lifante, José

AU - Ximendes, Erving

AU - Laurenti, Marco

AU - Méndez-González, Diego

AU - Melle, Sonia

AU - Calderón, Oscar G.

AU - López Cabarcos, Enrique

AU - Fernández, Nuria

AU - Chaves-Coira, Irene

AU - Lucena-Agell, Daniel

AU - Monge, Luis

AU - Mackenzie, Mark D.

AU - Marqués-Hueso, José

AU - Jones, Callum M. S.

AU - Jacinto, Carlos

AU - del Rosal, Blanca

AU - Kar, Ajoy K.

AU - Rubio-Retama, Jorge

AU - Jaque, Daniel

PY - 2020/6/10

Y1 - 2020/6/10

N2 - Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm-2) and doses (<0.5 mg kg-1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.

AB - Optical probes operating in the second near-infrared window (NIR-II, 1,000-1,700 nm), where tissues are highly transparent, have expanded the applicability of fluorescence in the biomedical field. NIR-II fluorescence enables deep-tissue imaging with micrometric resolution in animal models, but is limited by the low brightness of NIR-II probes, which prevents imaging at low excitation intensities and fluorophore concentrations. Here, we present a new generation of probes (Ag2S superdots) derived from chemically synthesized Ag2S dots, on which a protective shell is grown by femtosecond laser irradiation. This shell reduces the structural defects, causing an 80-fold enhancement of the quantum yield. PEGylated Ag2S superdots enable deep-tissue in vivo imaging at low excitation intensities (<10 mW cm-2) and doses (<0.5 mg kg-1), emerging as unrivaled contrast agents for NIR-II preclinical bioimaging. These results establish an approach for developing superbright NIR-II contrast agents based on the synergy between chemical synthesis and ultrafast laser processing.

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U2 - 10.1038/s41467-020-16333-2

DO - 10.1038/s41467-020-16333-2

M3 - Article

C2 - 32523065

SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 2933

ER -

Ultrafast photochemistry produces superbright short-wave infrared dots for low-dose in vivo imaging

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