TY - JOUR
T1 - Tuning Physical Properties of GelMA Hydrogels through Microarchitecture for Engineering Osteoid Tissue
AU - Walejewska, Ewa
AU - Melchels, Ferry P. W.
AU - Paradiso, Alessia
AU - McCormack, Andrew
AU - Szlazak, Karol
AU - Olszewska, Alicja
AU - Srebrzynski, Michal
AU - Swieszkowski, Wojciech
N1 - Funding Information:
This research was supported by grant no. UMO-2021/41/N/ST5/04220 from the Polish National Science Centre and by the “Excellence Initiative─Research University” at the Warsaw University of Technology (04/IDUB/2019/94) within the Mobility PW program. This work was also partially supported by the National Centre for Research and Development under grant no. POLTUR4/BIOCANCER/3/2021. A was partially created with BioRender.com . We would like to thank Maciej Łojkowski and Dr Emilia Choińska for their support with FTiR and TGA measurements.
Publisher Copyright:
© 2023 American Chemical Society
PY - 2024/1/8
Y1 - 2024/1/8
N2 - Gelatin methacryloyl (GelMA) hydrogels have gained significant attention due to their biocompatibility and tunable properties. Here, a new approach to engineer GelMA-based matrices to mimic the osteoid matrix is provided. Two cross-linking methods were employed to mimic the tissue stiffness: standard cross-linking (SC) based on visible light exposure (VL) and dual cross-linking (DC) involving physical gelation, followed by VL. It was demonstrated that by reducing the GelMA concentration from 10% (G10) to 5% (G5), the dual-cross-linked G5 achieved a compressive modulus of ∼17 kPa and showed the ability to support bone formation, as evidenced by alkaline phosphatase detection over 3 weeks of incubation in osteogenic medium. Moreover, incorporating poly(ethylene) oxide (PEO) into the G5 and G10 samples was found to hinder the fabrication of highly porous hydrogels, leading to compromised cell survival and reduced osteogenic differentiation, as a consequence of incomplete PEO removal.
AB - Gelatin methacryloyl (GelMA) hydrogels have gained significant attention due to their biocompatibility and tunable properties. Here, a new approach to engineer GelMA-based matrices to mimic the osteoid matrix is provided. Two cross-linking methods were employed to mimic the tissue stiffness: standard cross-linking (SC) based on visible light exposure (VL) and dual cross-linking (DC) involving physical gelation, followed by VL. It was demonstrated that by reducing the GelMA concentration from 10% (G10) to 5% (G5), the dual-cross-linked G5 achieved a compressive modulus of ∼17 kPa and showed the ability to support bone formation, as evidenced by alkaline phosphatase detection over 3 weeks of incubation in osteogenic medium. Moreover, incorporating poly(ethylene) oxide (PEO) into the G5 and G10 samples was found to hinder the fabrication of highly porous hydrogels, leading to compromised cell survival and reduced osteogenic differentiation, as a consequence of incomplete PEO removal.
UR - http://www.scopus.com/inward/record.url?scp=85181031302&partnerID=8YFLogxK
U2 - 10.1021/acs.biomac.3c00909
DO - 10.1021/acs.biomac.3c00909
M3 - Article
C2 - 38102990
AN - SCOPUS:85181031302
SN - 1525-7797
VL - 25
SP - 188
EP - 199
JO - Biomacromolecules
JF - Biomacromolecules
IS - 1
ER -