TY - JOUR
T1 - Tri-mode optical biopsy probe with fluorescence endomicroscopy, Raman spectroscopy, and time-resolved fluorescence spectroscopy
AU - Wood, Harry Alexander Charles
AU - Ehrlich, Katjana
AU - Yerolatsitis, Stephanos
AU - Kufcsák, András
AU - Quinn, Tom Michael
AU - Fernandes, Susan
AU - Norberg, Dominic
AU - Jenkins, Nia Caitlin
AU - Young, Vikki
AU - Young, Irene
AU - Hamilton, Katie
AU - Seth, Sohan
AU - Akram, Ahsan
AU - Thomson, Robert Rodrick
AU - Finlayson, Keith
AU - Dhaliwal, Kevin
AU - Stone, James Morgan
N1 - Funding Information:
This work was supported by the UKRI grants EP/S001123/1 and MR/R017794/1, and an award from BTG/Boston Scientific.
Publisher Copyright:
© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
PY - 2023/2
Y1 - 2023/2
N2 - We present an endoscopic probe that combines three distinct optical fibre technologies including: A high-resolution imaging fibre for optical endomicroscopy, a multimode fibre for time-resolved fluorescence spectroscopy, and a hollow-core fibre with multimode signal collection cores for Raman spectroscopy. The three fibers are all enclosed within a 1.2 mm diameter clinical grade catheter with a 1.4 mm end cap. To demonstrate the probe's flexibility we provide data acquired with it in loops of radii down to 2 cm. We then use the probe in an anatomically accurate model of adult human airways, showing that it can be navigated to any part of the distal lung using a commercial bronchoscope. Finally, we present data acquired from fresh ex vivo human lung tissue. Our experiments show that this minimally invasive probe can deliver real-time optical biopsies from within the distal lung - simultaneously acquiring co-located high-resolution endomicroscopy and biochemical spectra.
AB - We present an endoscopic probe that combines three distinct optical fibre technologies including: A high-resolution imaging fibre for optical endomicroscopy, a multimode fibre for time-resolved fluorescence spectroscopy, and a hollow-core fibre with multimode signal collection cores for Raman spectroscopy. The three fibers are all enclosed within a 1.2 mm diameter clinical grade catheter with a 1.4 mm end cap. To demonstrate the probe's flexibility we provide data acquired with it in loops of radii down to 2 cm. We then use the probe in an anatomically accurate model of adult human airways, showing that it can be navigated to any part of the distal lung using a commercial bronchoscope. Finally, we present data acquired from fresh ex vivo human lung tissue. Our experiments show that this minimally invasive probe can deliver real-time optical biopsies from within the distal lung - simultaneously acquiring co-located high-resolution endomicroscopy and biochemical spectra.
KW - Raman endoscopy
KW - fluorescence endomicroscopy
KW - micro endoscopy
KW - time-resolved fluorescence spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=85138439196&partnerID=8YFLogxK
U2 - 10.1002/jbio.202200141
DO - 10.1002/jbio.202200141
M3 - Article
C2 - 36062395
SN - 1864-063X
VL - 16
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 2
M1 - e202200141
ER -