Translation Microscopy (TRAM) for super-resolution imaging

Zhen Qiu, Rhodri Simon Wilson, Yuewei Liu, Alison Dun, Rebecca Sonia Saleeb, Dongsheng Liu, Colin Rickman, Margaret Frame, Rory Duncan, Weiping Lu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
61 Downloads (Pure)


Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ground-truth DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

Original languageEnglish
Article number19993
JournalScientific Reports
Publication statusPublished - 29 Jan 2016

ASJC Scopus subject areas

  • General


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