Translation Microscopy (TRAM) for super-resolution imaging

Zhen Qiu, Rhodri Simon Wilson, Yuewei Liu, Alison Dun, Rebecca Sonia Saleeb, Dongsheng Liu, Colin Rickman, Margaret Frame, Rory Duncan, Weiping Lu

Research output: Contribution to journalArticle

2 Citations (Scopus)
28 Downloads (Pure)

Abstract

Super-resolution microscopy is transforming our understanding of biology but accessibility is limited by its technical complexity, high costs and the requirement for bespoke sample preparation. We present a novel, simple and multi-color super-resolution microscopy technique, called translation microscopy (TRAM), in which a super-resolution image is restored from multiple diffraction-limited resolution observations using a conventional microscope whilst translating the sample in the image plane. TRAM can be implemented using any microscope, delivering up to 7-fold resolution improvement. We compare TRAM with other super-resolution imaging modalities, including gated stimulated emission deletion (gSTED) microscopy and atomic force microscopy (AFM). We further developed novel ground-truth DNA origami nano-structures to characterize TRAM, as well as applying it to a multi-color dye-stained cellular sample to demonstrate its fidelity, ease of use and utility for cell biology.

Original languageEnglish
Article number19993
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 29 Jan 2016

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Translation Microscopy (TRAM) for super-resolution imaging'. Together they form a unique fingerprint.

  • Cite this

    Qiu, Z., Wilson, R. S., Liu, Y., Dun, A., Saleeb, R. S., Liu, D., Rickman, C., Frame, M., Duncan, R., & Lu, W. (2016). Translation Microscopy (TRAM) for super-resolution imaging. Scientific Reports, 6, [19993]. https://doi.org/10.1038/srep19993