TRANSIENT EXPRESSION IN LEAF MESOPHYLL PROTOPLASTS OF ARABIDOPSIS-THALIANA

A HOFFMAN, U HALFTER, PC MORRIS

    Research output: Contribution to journalArticle

    Abstract

    Conditions for maximising transient expression of GUS in leaf mesophyll protoplasts of Arabidopsis thaliana ecotype C24 were investigated. It was found that the factors most influencing expression levels, with optimum levels in parenthesis, were plasmid DNA quantity (100 mug per 5 x 10(5) protoplasts), inclusion of carrier DNA (50 mug), PEG pH and amount (pH above 6, and total PEG concentration at least 9% w/w) and the topological form of the DNA. Linearised plasmid DNA with long flanking sequences 3' and 5' to the marker gene yielded the highest levels of GUS expression.

    Original languageEnglish
    Pages (from-to)53-58
    Number of pages6
    JournalPlant Cell, Tissue and Organ Culture
    Volume36
    Issue number1
    Publication statusPublished - Jan 1994

    Keywords

    • PROTOPLAST
    • TRANSFORMATION CONDITIONS
    • DIRECT GENE-TRANSFER
    • HYBRID GENES
    • CULTURES
    • PLANT-PROTOPLASTS
    • TRANSIENT EXPRESSION
    • GUS
    • ARABIDOPSIS-THALIANA

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