Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures

Kok-Gan Chan, Kumutha Priya, Chien-Yi Chang, Ahmad Yamin Abdul Rahman, Kok Keng Tee, Wai-Fong Yin

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    Abstract

    Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 °C) and an arbitrarily-selected temperature of 46 °C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 °C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products.
    Original languageEnglish
    Article numbere2223
    JournalPeerJ
    Volume4
    DOIs
    Publication statusPublished - 19 Jul 2016

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    Chan, K-G., Priya, K., Chang, C-Y., Rahman, A. Y. A., Tee, K. K., & Yin, W-F. (2016). Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures. PeerJ, 4, [e2223]. https://doi.org/10.7717/peerj.2223