Toxicity Profiling of Biosurfactants Produced by Novel Marine Bacterial Strains

Georgia-Persephoni Voulgaridou, Theodora Mantso, Ioannis Anestopoulos, Ariel Klavaris, Christina Katzastra, Despina-Eugenia Kiousi, Marini Mantela, Alex Galanis, Konstantinos Gardikis, Ibrahim M. Banat, Tony Gutierrez, Karina Salek, Stephen Euston, Mihalis I. Panayiotids, Aglaia Pappa

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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Abstract

Surface active agents (SAAs), currently used in modern industry, are synthetic chemicals produced from non-renewable sources, with potential toxic impacts on humans and the environment. Thus, there is an increased interest for the identification and utilization of natural derived SAAs. As such, the marine environment is considered a promising source of biosurfactants with low toxicity, environmental compatibility, and biodegradation compared to their synthetic counterparts. MARISURF is a Horizon 2020 EU-funded project aiming to identify and functionally characterize SAAs, derived from a unique marine bacterial collection, towards commercial exploitation. Specifically, rhamnolipids produced by Marinobacter MCTG107b and Pseudomonas MCTG214(3b1) strains were previously identified and characterized while currently their toxicity profile was assessed by utilizing well-established methodologies. Our results showed a lack of cytotoxicity in in vitro models of human skin and liver as indicated by alamar blue and propidium iodide assays. Additionally, the use of the single gel electrophoresis assay, under oxidative stress conditions, revealed absence of any significant mutagenic/anti-mutagenic potential. Finally, both 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) cell-free assays, revealed no significant anti-oxidant capacity for neither of the tested compounds. Consequently, the absence of significant cytotoxicity and/or mutagenicity justifies their commercial exploitation and potential development into industrial end-user applications as natural and environmentally friendly biosurfactants.
Original languageEnglish
Article number2383
JournalInternational Journal of Molecular Sciences
Volume22
Issue number5
Early online date27 Feb 2021
DOIs
Publication statusPublished - 1 Mar 2021

Keywords

  • Anti-mutagenic activity
  • Antioxidant activity
  • Cytotoxicity
  • In vitro liver model
  • In vitro skin model
  • Marine biosurfactants
  • Marinobacter strains
  • Pseudomonas strains
  • Surface active agents
  • Toxicity profiling

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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