Time-correlated single photon counting FLIM: Some considerations for physiologists

Claire N. Medine, Angela McDonald, Axel Bergmann, Rory Duncan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Recent developments in cellular imaging now permit the minimally invasive study of protein interactions in living cells. These advances are of enormous interest to cell biologists, as proteins rarely act in isolation, but rather in concert with others in forming cellular machinery. Up until recently, all protein interactions had to be determined in vitro using biochemical approaches. This biochemical legacy has provided cell biologists with the basis to test defined protein-protein interactions not only inside cells, but now also with spatial resolution. More recent developments in TCSPC imaging are now also driving towards being able to determine protein interaction rates with similar spatial resolution, and together, these experimental advances allow investigators to perform biochemical experiments inside living cells. Here, we discuss some findings we have made along the way which may be useful for physiologists to consider.

Original languageEnglish
Pages (from-to)420-425
Number of pages6
JournalMicroscopy Research and Technique
Volume70
Issue number5
DOIs
Publication statusPublished - May 2007

Keywords

  • Biology
  • Diagnostic Imaging
  • Fluorescence
  • Protein Binding
  • Proteins
  • Time Factors

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