The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit J. Karki; Paola Pilo; Colleen Lawless; Nikolaos  Mastrodimos; Anna M. M. Tiley; James I. Burke; Angela Feechan

doi:10.1371/journal.pone.0313859

The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

Sujit J. Karki, Paola Pilo, Colleen Lawless, Nikolaos Mastrodimos, Anna M. M. Tiley, James I. Burke, Angela Feechan

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Abstract

Zymoseptoria tritici is an ascomycete fungus and the causal agent of Septoria tritici leaf blotch (STB) in wheat. Z. tritici secretes an array of effector proteins that are likely to facilitate host infection, colonisation and pycnidia production. In this study we demonstrate a role for Zt-11 as a Z. tritici effector during disease progression. Zt-11 is upregulated during the transition of the pathogen from the biotrophic to necrotrophic phase of wheat infection. Deletion of Zt-11 delayed disease development in wheat, reducing the number and size of pycnidia, as well as the number of macropycnidiospores produced by Z. tritici. This delayed disease development by the ΔZt-11 mutants was accompanied by a lower induction of PR genes in wheat, when compared to infection with wildtype Z. tritici. Overall, these data suggest that Zt-11 plays a role in Z. tritici aggressiveness and STB disease progression possibly via a salicylic acid associated pathway.

Original language	English
Article number	e0313859
Journal	PLoS ONE
Volume	19
Issue number	11
DOIs	https://doi.org/10.1371/journal.pone.0313859
Publication status	Published - 19 Nov 2024

Keywords

Wheat
Leaves
Yeast
Mutant strains
Necrosis
Polymerase chain reaction
Gene expression
Plasmid construction

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abstract = "Zymoseptoria tritici is an ascomycete fungus and the causal agent of Septoria tritici leaf blotch (STB) in wheat. Z. tritici secretes an array of effector proteins that are likely to facilitate host infection, colonisation and pycnidia production. In this study we demonstrate a role for Zt-11 as a Z. tritici effector during disease progression. Zt-11 is upregulated during the transition of the pathogen from the biotrophic to necrotrophic phase of wheat infection. Deletion of Zt-11 delayed disease development in wheat, reducing the number and size of pycnidia, as well as the number of macropycnidiospores produced by Z. tritici. This delayed disease development by the ΔZt-11 mutants was accompanied by a lower induction of PR genes in wheat, when compared to infection with wildtype Z. tritici. Overall, these data suggest that Zt-11 plays a role in Z. tritici aggressiveness and STB disease progression possibly via a salicylic acid associated pathway.",

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AU - Mastrodimos, Nikolaos

AU - Tiley, Anna M. M.

AU - Burke, James I.

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AB - Zymoseptoria tritici is an ascomycete fungus and the causal agent of Septoria tritici leaf blotch (STB) in wheat. Z. tritici secretes an array of effector proteins that are likely to facilitate host infection, colonisation and pycnidia production. In this study we demonstrate a role for Zt-11 as a Z. tritici effector during disease progression. Zt-11 is upregulated during the transition of the pathogen from the biotrophic to necrotrophic phase of wheat infection. Deletion of Zt-11 delayed disease development in wheat, reducing the number and size of pycnidia, as well as the number of macropycnidiospores produced by Z. tritici. This delayed disease development by the ΔZt-11 mutants was accompanied by a lower induction of PR genes in wheat, when compared to infection with wildtype Z. tritici. Overall, these data suggest that Zt-11 plays a role in Z. tritici aggressiveness and STB disease progression possibly via a salicylic acid associated pathway.

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The Zymoseptoria tritici effector Zt-11 contributes to aggressiveness in wheat

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