TY - JOUR
T1 - The validity of Western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of Renibacterium salmoninarum
AU - McIntosh, D.
AU - Austin, B.
PY - 1996/6
Y1 - 1996/6
N2 - Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.
AB - Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.
KW - 57 kDa antigen
KW - Cross-reactivity
KW - Enhanced chemiluminescence
KW - Renibacterium salmoninarum
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=0030174810&partnerID=8YFLogxK
U2 - 10.1016/0167-7012(96)00004-8
DO - 10.1016/0167-7012(96)00004-8
M3 - Article
SN - 0167-7012
VL - 25
SP - 329
EP - 335
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 3
ER -