The validity of Western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of Renibacterium salmoninarum

D. McIntosh; B. Austin

doi:10.1016/0167-7012(96)00004-8

The validity of Western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of Renibacterium salmoninarum

D. McIntosh, B. Austin

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.

Original language	English
Pages (from-to)	329-335
Number of pages	7
Journal	Journal of Microbiological Methods
Volume	25
Issue number	3
DOIs	https://doi.org/10.1016/0167-7012(96)00004-8
Publication status	Published - Jun 1996

Keywords

57 kDa antigen
Cross-reactivity
Enhanced chemiluminescence
Renibacterium salmoninarum
Western blotting

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10.1016/0167-7012(96)00004-8

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title = "The validity of Western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of Renibacterium salmoninarum",

abstract = "Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.",

keywords = "57 kDa antigen, Cross-reactivity, Enhanced chemiluminescence, Renibacterium salmoninarum, Western blotting",

author = "D. McIntosh and B. Austin",

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AU - McIntosh, D.

AU - Austin, B.

PY - 1996/6

Y1 - 1996/6

N2 - Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.

AB - Six strains of Renibacterium salmoninarum were examined by Western blotting, which incorporated polyclonal and monoclonal antisera produced against the 57 kDa surface protein (=p57 antigen). An enhanced chemiluminescence (ECL) detection system increased the sensitivity of the reaction when compared to the use of chromogenic substrates. However, Western blotting was not as sensitive as culturing methods for the detection of R. salmoninarum during early stages of infection in salmonids. Yet, Western blotting was determined to be specific to R. salmoninarum, insofar as cross- reactions did not occur with Corynebacterium aquaticum or Carnobacterium piscicola.

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KW - Cross-reactivity

KW - Enhanced chemiluminescence

KW - Renibacterium salmoninarum

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DO - 10.1016/0167-7012(96)00004-8

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VL - 25

SP - 329

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

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ER -

The validity of Western blotting for the diagnosis of bacterial kidney disease based on the detection of the p57 antigen of Renibacterium salmoninarum

Abstract

Keywords

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