The Trimeric Autotransporter Adhesin YadA of Yersinia enterocolitica Serotype O:9 Binds Glycan Moieties

Ina Meuskens, Juan Leva-Bueno, Paul Millner, Monika Schütz, Sally A. Peyman, Dirk Linke*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)
1 Downloads (Pure)

Abstract

Yersinia adhesin A (YadA) is a key virulence factor of Yersinia enterocolitica and Yersinia pseudotuberculosis. YadA is a trimeric autotransporter adhesin, a class of adhesins that have been shown to enable many Gram-negative pathogens to adhere to/interact with the host extracellular matrix proteins such as collagen, vitronectin, and fibronectin. Here, we show for the first time that YadA of Yersinia enterocolitica serotype O:9 not only interacts with proteinaceous surface molecules but can also attach directly to glycan moieties. We show that YadA from Y. enterocolitica serotype O:9 does not interact with the vitronectin protein itself but exclusively with its N-linked glycans. We also show that YadA can target other glycan moieties as found in heparin, for example. So far, little is known about specific interactions between bacterial autotransporter adhesins and glycans. This could potentially lead to new antimicrobial treatment strategies, as well as diagnostic applications.

Original languageEnglish
Article number738818
JournalFrontiers in Microbiology
Volume12
DOIs
Publication statusPublished - 1 Feb 2022

Keywords

  • adhesion
  • bacterial adhesion
  • extracellular matrix (ECM)
  • glycan
  • trimeric autotransporter adhesin
  • virulence

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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