The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

A. P. Bradshaw; R. J. Sturgeon

doi:10.1007/BF00156613

The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

A. P. Bradshaw, R. J. Sturgeon

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. © 1990 Science & Technology Letters.

Original language	English
Pages (from-to)	67-71
Number of pages	5
Journal	Biotechnology Techniques
Volume	4
Issue number	1
DOIs	https://doi.org/10.1007/BF00156613
Publication status	Published - Jan 1990

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10.1007/BF00156613

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title = "The synthesis of soluble polymer-ligand complexes for affinity precipitation studies",

abstract = "Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. {\textcopyright} 1990 Science \& Technology Letters.",

author = "Bradshaw, \{A. P.\} and Sturgeon, \{R. J.\}",

year = "1990",

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AU - Bradshaw, A. P.

AU - Sturgeon, R. J.

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N2 - Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. © 1990 Science & Technology Letters.

AB - Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. © 1990 Science & Technology Letters.

UR - https://www.scopus.com/pages/publications/0037860427

U2 - 10.1007/BF00156613

DO - 10.1007/BF00156613

M3 - Article

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VL - 4

SP - 67

EP - 71

JO - Biotechnology Techniques

JF - Biotechnology Techniques

IS - 1

ER -

The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

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