The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

A. P. Bradshaw, R. J. Sturgeon

    Research output: Contribution to journalArticle

    Abstract

    Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. © 1990 Science & Technology Letters.

    Original languageEnglish
    Pages (from-to)67-71
    Number of pages5
    JournalBiotechnology Techniques
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - Jan 1990

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    Staphylococcal Protein A
    Polysaccharides
    Polymers
    Bearings (structural)
    Galactose Oxidase
    Ligands
    Borates
    Galactose
    Aldehydes
    Immunoglobulins
    Immunoglobulin G
    Recovery
    Oxidation
    Water
    galactomannan

    Cite this

    Bradshaw, A. P. ; Sturgeon, R. J. / The synthesis of soluble polymer-ligand complexes for affinity precipitation studies. In: Biotechnology Techniques. 1990 ; Vol. 4, No. 1. pp. 67-71.
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    The synthesis of soluble polymer-ligand complexes for affinity precipitation studies. / Bradshaw, A. P.; Sturgeon, R. J.

    In: Biotechnology Techniques, Vol. 4, No. 1, 01.1990, p. 67-71.

    Research output: Contribution to journalArticle

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