The synthesis of soluble polymer-ligand complexes for affinity precipitation studies

A. P. Bradshaw, R. J. Sturgeon

    Research output: Contribution to journalArticle

    Abstract

    Protein A has been covalently coupled to a water soluble galactomannan bearing aldehyde groups, introduced by oxidation with D-galactose oxidase. The soluble conjugate has been used to form complexes with immunoglobulin G, precipitation being induced by the formation of polysaccharide-borate complexes. After recovery of the immunoglobulin, the polysaccharide-protein A conjugates can be re-cycled and used for further isolations. © 1990 Science & Technology Letters.

    Original languageEnglish
    Pages (from-to)67-71
    Number of pages5
    JournalBiotechnology Techniques
    Volume4
    Issue number1
    DOIs
    Publication statusPublished - Jan 1990

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