The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein

S. Zhao; N. W. Douglas; M. J S Heine; G. M. Williams; H. C. Winther-Larsen; P. G. Meaden

doi:10.1016/0378-1119(94)90295-X

The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein

S. Zhao, N. W. Douglas, M. J S Heine, G. M. Williams, H. C. Winther-Larsen, P. G. Meaden

Research output: Contribution to journal › Article › peer-review

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Abstract

A gene has been cloned from the yeast Saccharomyces cerevisiae which, on the basis of the deduced translation product, encodes a sugar transporter-like protein. This gene, STL1, was identified as an open reading frame (ORF) closely linked to the cinnamic-acid-resistance gene POF1 on chromosome IV. The putative translation product of STL1 (STLl) contains 536 amino acids, with a M(r) of 60 507. Hydropathy analysis of STL1 suggests that it contains the twelve transmembrane (TM) domains characteristic of a family of sugar transporters from S. cerevisiae and other organisms. STL1 displays greatest homology (28% identity) to the products of the yeast HXT2 (hexose transporter) and GAL2 (galactose transporter) genes. Disruption of STL1 had no detectable effect on yeast growth on glucose, galactose, mannose, maltose or glycerol as sole carbon source. The transport function of the gene product remains unknown at present.

Original language	English
Pages (from-to)	215-219
Number of pages	5
Journal	Gene
Volume	146
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)90295-X
Publication status	Published - 1994

Keywords

Cinnamic acid resistance
Cloning
Dna sequencing
Gene disruption
Gene families
Hybridization

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10.1016/0378-1119(94)90295-X

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title = "The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein",

abstract = "A gene has been cloned from the yeast Saccharomyces cerevisiae which, on the basis of the deduced translation product, encodes a sugar transporter-like protein. This gene, STL1, was identified as an open reading frame (ORF) closely linked to the cinnamic-acid-resistance gene POF1 on chromosome IV. The putative translation product of STL1 (STLl) contains 536 amino acids, with a M(r) of 60 507. Hydropathy analysis of STL1 suggests that it contains the twelve transmembrane (TM) domains characteristic of a family of sugar transporters from S. cerevisiae and other organisms. STL1 displays greatest homology (28% identity) to the products of the yeast HXT2 (hexose transporter) and GAL2 (galactose transporter) genes. Disruption of STL1 had no detectable effect on yeast growth on glucose, galactose, mannose, maltose or glycerol as sole carbon source. The transport function of the gene product remains unknown at present.",

keywords = "Cinnamic acid resistance, Cloning, Dna sequencing, Gene disruption, Gene families, Hybridization",

author = "S. Zhao and Douglas, {N. W.} and Heine, {M. J S} and Williams, {G. M.} and Winther-Larsen, {H. C.} and Meaden, {P. G.}",

year = "1994",

doi = "10.1016/0378-1119(94)90295-X",

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T1 - The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein

AU - Zhao, S.

AU - Douglas, N. W.

AU - Heine, M. J S

AU - Williams, G. M.

AU - Winther-Larsen, H. C.

AU - Meaden, P. G.

PY - 1994

Y1 - 1994

N2 - A gene has been cloned from the yeast Saccharomyces cerevisiae which, on the basis of the deduced translation product, encodes a sugar transporter-like protein. This gene, STL1, was identified as an open reading frame (ORF) closely linked to the cinnamic-acid-resistance gene POF1 on chromosome IV. The putative translation product of STL1 (STLl) contains 536 amino acids, with a M(r) of 60 507. Hydropathy analysis of STL1 suggests that it contains the twelve transmembrane (TM) domains characteristic of a family of sugar transporters from S. cerevisiae and other organisms. STL1 displays greatest homology (28% identity) to the products of the yeast HXT2 (hexose transporter) and GAL2 (galactose transporter) genes. Disruption of STL1 had no detectable effect on yeast growth on glucose, galactose, mannose, maltose or glycerol as sole carbon source. The transport function of the gene product remains unknown at present.

AB - A gene has been cloned from the yeast Saccharomyces cerevisiae which, on the basis of the deduced translation product, encodes a sugar transporter-like protein. This gene, STL1, was identified as an open reading frame (ORF) closely linked to the cinnamic-acid-resistance gene POF1 on chromosome IV. The putative translation product of STL1 (STLl) contains 536 amino acids, with a M(r) of 60 507. Hydropathy analysis of STL1 suggests that it contains the twelve transmembrane (TM) domains characteristic of a family of sugar transporters from S. cerevisiae and other organisms. STL1 displays greatest homology (28% identity) to the products of the yeast HXT2 (hexose transporter) and GAL2 (galactose transporter) genes. Disruption of STL1 had no detectable effect on yeast growth on glucose, galactose, mannose, maltose or glycerol as sole carbon source. The transport function of the gene product remains unknown at present.

KW - Cinnamic acid resistance

KW - Cloning

KW - Dna sequencing

KW - Gene disruption

KW - Gene families

KW - Hybridization

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DO - 10.1016/0378-1119(94)90295-X

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VL - 146

SP - 215

EP - 219

JO - Gene

JF - Gene

IS - 2

ER -

The STL1 gene of Saccharomyces cerevisiae is predicted to encode a sugar transporter-like protein

Abstract

Keywords

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