The stabilty and turnover of the maltose transporter of yeast: Epitope tagging of the MAL6/ gene product

P. W. Dalgleish, P. G. Meaden, J. C. Slaughter

    Research output: Contribution to journalArticle

    Abstract

    The regulated uptake of wort sugars (especially maltose) by yeast is a prime determinant of beer fermentation progress. Under some nutrient-limited conditions the ability to take up sugars declines markedly so fermentation is re strained or even ceases. In order to gain a better understanding of the molecular mechanisms involved in control of maltose uptake in yeast a method for the detection and measurement of the maltose transporter in yeast cells has been developed. The maltose transporter of yeast has been tagged with a short epitope at its C terminal end by incorporating the DNA sequence encoding the epitope into the maltose transporter gene by PCR mutagenesis. Antibodies to the epitope are commercially available. It has been demonstrated that the addition of the epitope has no effect on the functional activity of the protein. The modified protein is abundantly expressed and localised to the cell membrane. The relationship between the amount of the transporter in the cell membrane and its activity under fermentation conditions is being explored. PWD acknowledge award of a BBSRC CASE studentship supported by Whitbread plc.

    Original languageEnglish
    Pages (from-to)A1073
    JournalFASEB Journal: : The Journal of the Federation of American Societies for Experimental Biology
    Volume11
    Issue number9
    Publication statusPublished - 1997

    Fingerprint Dive into the research topics of 'The stabilty and turnover of the maltose transporter of yeast: Epitope tagging of the MAL6/ gene product'. Together they form a unique fingerprint.

    Cite this