The potential of confocal imaging for measuring physiological changes in brewer's yeast

Cornelia Schlee; Michaela Miedl; Kenneth A. Leiper; Graham G. Stewart

The potential of confocal imaging for measuring physiological changes in brewer's yeast

Cornelia Schlee, Michaela Miedl, Kenneth A. Leiper, Graham G. Stewart

Research output: Contribution to journal › Article › peer-review

Abstract

This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation. © 2006 The Institute of Brewing & Distilling.

Original language	English
Pages (from-to)	134-147
Number of pages	14
Journal	Journal of the Institute of Brewing
Volume	112
Issue number	2
Publication status	Published - 2006

Keywords

Brewing
Confocal imaging
Fluorescence microscopy
Glycogen
Proteinase
Trehalose
Yeast

Cite this

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title = "The potential of confocal imaging for measuring physiological changes in brewer's yeast",

abstract = "This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation. {\textcopyright} 2006 The Institute of Brewing & Distilling.",

keywords = "Brewing, Confocal imaging, Fluorescence microscopy, Glycogen, Proteinase, Trehalose, Yeast",

author = "Cornelia Schlee and Michaela Miedl and Leiper, {Kenneth A.} and Stewart, {Graham G.}",

year = "2006",

language = "English",

volume = "112",

pages = "134--147",

journal = "Journal of the Institute of Brewing",

issn = "0046-9750",

publisher = "Chartered Institute of Brewers and Distillers",

number = "2",

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TY - JOUR

T1 - The potential of confocal imaging for measuring physiological changes in brewer's yeast

AU - Schlee, Cornelia

AU - Miedl, Michaela

AU - Leiper, Kenneth A.

AU - Stewart, Graham G.

PY - 2006

Y1 - 2006

N2 - This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation. © 2006 The Institute of Brewing & Distilling.

AB - This study focused on the implementation of fluorescence optical methods and laser scanning confocal microscopy for monitoring brewing yeast performance. Physiological parameters and cell compounds in yeast cells (glycogen, neutral lipids, trehalose, bud scars, DNA and intracellular proteinases) have been successfully visualised with the aid of highly specific fluorochromes. The expression and sub cellular localisation of proteinase A during fermentation has been studied employing a Saccharomyces cerevisiae green fluorescent protein clone. This novel approach to monitoring brewing yeast performance provides new insights into physiological events that occur during wort fermentation. © 2006 The Institute of Brewing & Distilling.

KW - Brewing

KW - Confocal imaging

KW - Fluorescence microscopy

KW - Glycogen

KW - Proteinase

KW - Trehalose

KW - Yeast

M3 - Article

SN - 0046-9750

VL - 112

SP - 134

EP - 147

JO - Journal of the Institute of Brewing

JF - Journal of the Institute of Brewing

IS - 2

ER -

The potential of confocal imaging for measuring physiological changes in brewer's yeast

Abstract

Keywords

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