The photosynthetic characteristics of red coralline algae, determined using pulse amplitude modulation (PAM) fluorometry

Heidi Burdett, Sebastian J. Hennige, Fiona T-Y. Francis, Nicholas A. Kamenos

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17 Citations (Scopus)

Abstract

Interest in red coralline algae is increasing due to their projected sensitivity to ocean acidification and their utility as palaeoenvironmental proxies. Thus, it is crucial to obtain a thorough understanding of their basic photosynthetic characteristics and appropriate techniques for use in both laboratory and in situ studies. This study provides fluorescence methodology and data for the ecologically important red coralline alga Lithothamnion glaciale using pulse amplitude modulation (PAM) fluorometry. Lithothamnion glaciale was sufficiently dark-acclimated for in situ work following 10 s of quasi-darkness, attaining 95-98% of the maximum photochemical efficiency (F-v/F-m). Rapid light curves conducted in situ and in the laboratory determined a low light adaptation, with a saturation intensity of 4.45-54.6 mu mol photons m(-2)s(-1). Intra-thallus heterogeneity was observed between branch tips and bases (i.e., within the thallus) using a custom-made 2 mm fibre optic probe (the heterogeneity could not be detected using the standard 5 mm probe). Branch bases were lower light acclimated than the tips, with higher maximum effective quantum yield (F-q'/F-m'(max)) and lower non-photochemical quenching. Samples measured in May were higher light acclimated than in March, which suggests a degree of seasonal acclimation. Light history and photon irradiance levels were thus found to significantly affect the photosynthetic characteristics of L. glaciale

Original languageEnglish
Pages (from-to)499-509
Number of pages11
JournalBotanica Marina
Volume55
Issue number5
DOIs
Publication statusPublished - Oct 2012

Keywords

  • PHOTOSYNTHESIS
  • RAPID LIGHT CURVES
  • MAERL
  • MACROALGAE
  • ELECTRON-TRANSPORT
  • CORALLINE ALGAE (CCA)
  • RHODOPHYTA
  • CRUSTOSE
  • NORTHEAST ATLANTIC
  • RHODOLITH
  • CHLOROPHYLL FLUORESCENCE
  • PHOTOINHIBITION
  • GROWTH
  • FLUORESCENCE MEASUREMENTS
  • LIGHT

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