Abstract
The photochemical stability of collagen–chitosan blends in solution and film form was investigated using viscosimetry measurements,
UV-Vis spectrophotometry, FTIR spectroscopy and wide angle X-ray diffraction. It was found that the relative viscosity of collagen
decreased upon UV irradiation. The initial relative viscosity of collagen–chitosan blends were greater than the viscosity of collagen; upon
UV irradiation the viscosity of the blends decreased rapidly. The absorption/scattering of collagen in solution increased during irradiation
of the sample as shown by UV-Vis, indicating a conformational transition in the sample. FTIR showed that the amide A, B, I and II bands
from collagen are shifted after UV irradiation to lower wave numbers; these shifts in collagen–chitosan blends are less well pronounced.
Wide angle X-ray diffraction indicated that collagen and collagen–chitosan blends in film form retain much of their structural characteristics
after irradiation.
The viscosimetry andUV-Vis spectrophotometry results have shownthat solutions of collagen–chitosan blends are less stable photochemically
than a pure collagen solution. FTIR spectra have shown, that collagen–chitosan blended films are also less stable photochemically
than pure collagen films. Wide angle X-ray diffraction indicates that collagen and collagen–chitosan blend samples in film form are less
susceptible to conformational change than equivalent samples in solution.
UV-Vis spectrophotometry, FTIR spectroscopy and wide angle X-ray diffraction. It was found that the relative viscosity of collagen
decreased upon UV irradiation. The initial relative viscosity of collagen–chitosan blends were greater than the viscosity of collagen; upon
UV irradiation the viscosity of the blends decreased rapidly. The absorption/scattering of collagen in solution increased during irradiation
of the sample as shown by UV-Vis, indicating a conformational transition in the sample. FTIR showed that the amide A, B, I and II bands
from collagen are shifted after UV irradiation to lower wave numbers; these shifts in collagen–chitosan blends are less well pronounced.
Wide angle X-ray diffraction indicated that collagen and collagen–chitosan blends in film form retain much of their structural characteristics
after irradiation.
The viscosimetry andUV-Vis spectrophotometry results have shownthat solutions of collagen–chitosan blends are less stable photochemically
than a pure collagen solution. FTIR spectra have shown, that collagen–chitosan blended films are also less stable photochemically
than pure collagen films. Wide angle X-ray diffraction indicates that collagen and collagen–chitosan blend samples in film form are less
susceptible to conformational change than equivalent samples in solution.
| Original language | English |
|---|---|
| Pages (from-to) | 545-554 |
| Number of pages | 10 |
| Journal | Journal of Photochemistry and Photobiology A: Chemistry |
| Volume | 162 |
| Issue number | 2-3 |
| DOIs | |
| Publication status | Published - 15 Mar 2004 |