The location of tetrasaccharide units in pullulan

Gerard Carolan, Brian J. Catley, Fiona J McDougal

    Research output: Contribution to journalArticle

    Abstract

    The structure of pullulan, the extracellular a-d-glucan elaborated by the yeast-like fungus Aureobasidium pullulans, may be described as a polymaltotriose, the trisaccharides being linked by (1?6)-a-d-glucosidic bonds on the terminal d-glucosyl residues. Depending on the strain of the organism used, up to 6% of the trisaccharide units are replaced by maltotetraosyl residues. Exploiting the susceptibility of these tetrasaccharides to hydrolysis catalysed by porcine alpha-amylase, pullulan has been cleaved at these points and the fragments have been fractionated by gel-permeation chromatography. The heterogeneous size of the fragments indicates that there is no apparent, regular distribution of maltotetraosyl residues throughout the glucan. © 1983.

    Original languageEnglish
    Pages (from-to)237-243
    Number of pages7
    JournalCarbohydrate Research
    Volume114
    Issue number2
    Publication statusPublished - 1 Apr 1983

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    Trisaccharides
    Glucans
    alpha-Amylases
    Gel permeation chromatography
    Fungi
    Yeast
    Hydrolysis
    pullulan

    Cite this

    Carolan, G., Catley, B. J., & McDougal, F. J. (1983). The location of tetrasaccharide units in pullulan. Carbohydrate Research, 114(2), 237-243.
    Carolan, Gerard ; Catley, Brian J. ; McDougal, Fiona J. / The location of tetrasaccharide units in pullulan. In: Carbohydrate Research. 1983 ; Vol. 114, No. 2. pp. 237-243.
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    Carolan, G, Catley, BJ & McDougal, FJ 1983, 'The location of tetrasaccharide units in pullulan', Carbohydrate Research, vol. 114, no. 2, pp. 237-243.

    The location of tetrasaccharide units in pullulan. / Carolan, Gerard; Catley, Brian J.; McDougal, Fiona J.

    In: Carbohydrate Research, Vol. 114, No. 2, 01.04.1983, p. 237-243.

    Research output: Contribution to journalArticle

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    Carolan G, Catley BJ, McDougal FJ. The location of tetrasaccharide units in pullulan. Carbohydrate Research. 1983 Apr 1;114(2):237-243.