A limit dextrinase has been isolated from broad-bean flour and purified 800-fold. The enzyme readily hydrolysed branched a-dextrins containing maltosyl or maltotriosyl side-chains, pullulan, and amylopectin ß-limit dextrin. Glycogen ß-limit dextrins and amylopectin were slowly hydrolysed, but glycogens were barely susceptible to hydrolysis. There was no evidence for the presence of a second debranching enzyme in broad beans. © 1975.
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|Published - Jun 1975