The inhibition of bands 5 and 1 isoenzymes of porcine lactate dehydrogenase by salicylate was investigated kinetically. These studies indicated that up to two molecules of salicylate could combine with the active site of the free band 5 isoenzyme, and that one of the two salicylate molecules competes with its AMP binding site. It is concluded that salicylate competes with the AMP moiety of NAD+ in this enzyme.
|Number of pages||7|
|Journal||International Journal of Biochemistry|
|Publication status||Published - 1977|