The elasto-plastic nano- and microscale compressive behaviour of rehydrated mineralised collagen fibres

Alexander Groetsch, Aurélien Gourrier, Daniele Casari, Jakob Schwiedrzik, Jonathan D. Shephard, Johann Michler, Philippe K. Zysset, Uwe Wolfram

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Abstract

The hierarchical design of bio-based nanostructured materials such as bone enables them to combine unique structure-mechanical properties. As one of its main components, water plays an important role in bone's material multiscale mechanical interplay. However, its influence has not been quantified at the length-scale of a mineralised collagen fibre. Here, we couple in situ micropillar compression, and simultaneous synchrotron small angle X-ray scattering (SAXS) and X-ray diffraction (XRD) with a statistical constitutive model. Since the synchrotron data contain statistical information on the nanostructure, we establish a direct connection between experiment and model to identify the rehydrated elasto-plastic micro- and nanomechanical fibre behaviour. Rehydration led to a decrease of 65%-75% in fibre yield stress and compressive strength, and 70% in stiffness with a 3x higher effect on stresses than strains. While in agreement with bone extracellular matrix, the decrease is 1.5-3x higher compared to micro-indentation and macro-compression. Hydration influences mineral more than fibril strain with the highest difference to the macroscale when comparing mineral and tissue levels. The effect of hydration seems to be strongly mediated by ultrastructural interfaces while results provide insights towards mechanical consequences of reported water-mediated structuring of bone apatite. The missing reinforcing capacity of surrounding tissue for an excised fibril array is more pronounced in wet than dry conditions, mainly related to fibril swelling. Differences leading to higher compressive strength between mineralised tissues seem not to depend on rehydration while the lack of kink bands supports the role of water as an elastic embedding influencing energy-absorption mechanisms.
Original languageEnglish
Pages (from-to)332-345
Number of pages14
JournalActa Biomaterialia
Volume164
Early online date13 Apr 2023
DOIs
Publication statusPublished - 1 Jul 2023

Keywords

  • In situ synchrotron SAXS/XRD
  • Micropillar compression
  • Mineralised collagen fibre
  • Nano- and micromechanics
  • Rehydration
  • Statistical elasto-plastic constitutive model
  • Strength and failure

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Biochemistry
  • Biomedical Engineering
  • Molecular Biology

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