The determination of physostigmine by thin layer chromatography and ultraviolet spectrophotometry

A R Rogers, G Smith

Research output: Contribution to journalArticle

Abstract

Physostigmine has been separated from its degradation products by thin layer chromatography on alumina, with chloroform acetone (5:4) as the solvent for development. The alkaloid was eluted with methanolic hydrochloric acid and determined by ultraviolet spectrophotometry. Two methods were used for the correction of irrelevant absorbance: a differential method in which absorbance measurements were made at three wavelengths, and a method in which orthogonal functions were applied to absorbance measurements at a set of nine wavelengths.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume87
Issue number1
Publication statusPublished - 1973

Fingerprint Dive into the research topics of 'The determination of physostigmine by thin layer chromatography and ultraviolet spectrophotometry'. Together they form a unique fingerprint.

Cite this