Abstract
Physostigmine has been separated from its degradation products by thin layer chromatography on alumina, with chloroform acetone (5:4) as the solvent for development. The alkaloid was eluted with methanolic hydrochloric acid and determined by ultraviolet spectrophotometry. Two methods were used for the correction of irrelevant absorbance: a differential method in which absorbance measurements were made at three wavelengths, and a method in which orthogonal functions were applied to absorbance measurements at a set of nine wavelengths.
| Original language | English |
|---|---|
| Pages (from-to) | 125-136 |
| Number of pages | 12 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 87 |
| Issue number | 1 |
| Publication status | Published - 1973 |