Physostigmine has been separated from its degradation products by thin layer chromatography on alumina, with chloroform acetone (5:4) as the solvent for development. The alkaloid was eluted with methanolic hydrochloric acid and determined by ultraviolet spectrophotometry. Two methods were used for the correction of irrelevant absorbance: a differential method in which absorbance measurements were made at three wavelengths, and a method in which orthogonal functions were applied to absorbance measurements at a set of nine wavelengths.
|Number of pages||12|
|Journal||Journal of Chromatography B: Biomedical Sciences and Applications|
|Publication status||Published - 1973|