The cyst wall carbohydrate composition of Balamuthia mandrillaris

Ruqaiyyah Siddiqui; Naveed Ahmed Khan; Edward L. Jarroll

doi:10.1007/s00436-009-1346-8

The cyst wall carbohydrate composition of Balamuthia mandrillaris

Ruqaiyyah Siddiqui, Naveed Ahmed Khan^*, Edward L. Jarroll

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Balamuthia mandrillaris is an opportunistic cyst-producing amoeba that can cause rare, but fatal, Balamuthia amoebic encephalitis (BAE). Cysts are resistant to harsh environmental conditions and many antimicrobial compounds and thus can contribute to BAE recurrence. However, little is known of cyst wall synthesis, cyst wall composition, or how encystment is induced. In this study, we examined the carbohydrate composition of the cyst wall. The major components were mannose (20.9 mol%) and glucose (79.1 mol%), with trace amounts of galactose present in the cyst wall samples analysed. The linkage analysis showed cyst wall carbohydrates with apparently linear and branching saccharides and suggested the presence of cellulose. These components may play an important protective role by creating a permeability barrier around the cyst.

Original language	English
Pages (from-to)	1439–1443
Number of pages	5
Journal	Parasitology Research
Volume	104
DOIs	https://doi.org/10.1007/s00436-009-1346-8
Publication status	Published - May 2009

Keywords

Sodium Dodecyl Sulfate
Cyst Wall
Human Brain Microvascular Endothelial Cell
Methyl Glycoside
Intact Cyst

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10.1007/s00436-009-1346-8

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title = "The cyst wall carbohydrate composition of Balamuthia mandrillaris",

abstract = "Balamuthia mandrillaris is an opportunistic cyst-producing amoeba that can cause rare, but fatal, Balamuthia amoebic encephalitis (BAE). Cysts are resistant to harsh environmental conditions and many antimicrobial compounds and thus can contribute to BAE recurrence. However, little is known of cyst wall synthesis, cyst wall composition, or how encystment is induced. In this study, we examined the carbohydrate composition of the cyst wall. The major components were mannose (20.9 mol%) and glucose (79.1 mol%), with trace amounts of galactose present in the cyst wall samples analysed. The linkage analysis showed cyst wall carbohydrates with apparently linear and branching saccharides and suggested the presence of cellulose. These components may play an important protective role by creating a permeability barrier around the cyst.",

keywords = "Sodium Dodecyl Sulfate, Cyst Wall, Human Brain Microvascular Endothelial Cell, Methyl Glycoside, Intact Cyst",

author = "Ruqaiyyah Siddiqui and Khan, {Naveed Ahmed} and Jarroll, {Edward L.}",

year = "2009",

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doi = "10.1007/s00436-009-1346-8",

language = "English",

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journal = "Parasitology Research",

issn = "0932-0113",

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TY - JOUR

T1 - The cyst wall carbohydrate composition of Balamuthia mandrillaris

AU - Siddiqui, Ruqaiyyah

AU - Khan, Naveed Ahmed

AU - Jarroll, Edward L.

PY - 2009/5

Y1 - 2009/5

N2 - Balamuthia mandrillaris is an opportunistic cyst-producing amoeba that can cause rare, but fatal, Balamuthia amoebic encephalitis (BAE). Cysts are resistant to harsh environmental conditions and many antimicrobial compounds and thus can contribute to BAE recurrence. However, little is known of cyst wall synthesis, cyst wall composition, or how encystment is induced. In this study, we examined the carbohydrate composition of the cyst wall. The major components were mannose (20.9 mol%) and glucose (79.1 mol%), with trace amounts of galactose present in the cyst wall samples analysed. The linkage analysis showed cyst wall carbohydrates with apparently linear and branching saccharides and suggested the presence of cellulose. These components may play an important protective role by creating a permeability barrier around the cyst.

AB - Balamuthia mandrillaris is an opportunistic cyst-producing amoeba that can cause rare, but fatal, Balamuthia amoebic encephalitis (BAE). Cysts are resistant to harsh environmental conditions and many antimicrobial compounds and thus can contribute to BAE recurrence. However, little is known of cyst wall synthesis, cyst wall composition, or how encystment is induced. In this study, we examined the carbohydrate composition of the cyst wall. The major components were mannose (20.9 mol%) and glucose (79.1 mol%), with trace amounts of galactose present in the cyst wall samples analysed. The linkage analysis showed cyst wall carbohydrates with apparently linear and branching saccharides and suggested the presence of cellulose. These components may play an important protective role by creating a permeability barrier around the cyst.

KW - Sodium Dodecyl Sulfate

KW - Cyst Wall

KW - Human Brain Microvascular Endothelial Cell

KW - Methyl Glycoside

KW - Intact Cyst

U2 - 10.1007/s00436-009-1346-8

DO - 10.1007/s00436-009-1346-8

M3 - Article

C2 - 19214576

SN - 0932-0113

VL - 104

SP - 1439

EP - 1443

JO - Parasitology Research

JF - Parasitology Research

ER -

The cyst wall carbohydrate composition of Balamuthia mandrillaris

Abstract

Keywords

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