The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability

Eziuche Amadike Ugbogu, Sonja Ursula Wippler, Matthew Euston, Evelyn N Kouwenhoven, Arjan P M de Brouwer , Lilian Mary Schweizer, Michael Schweizer

    Research output: Contribution to journalArticle

    Abstract

    The gene products of the five-membered PRS gene family in Saccharomyces
    cerevisiae have been shown to exist as three minimal functional entities, Prs1/
    Prs3, Prs2/Prs5, and Prs4/Prs5, each capable of supporting cell viability. The
    Prs1/Prs3 heterodimer can be regarded as the most important because its loss
    causes temperature sensitivity. It has been shown that the GFP signal generated
    by an integrated GFP-Prs1 construct is lost in the absence of Prs3. In addition
    to interacting with Prs3, Prs1 also interacts with Slt2, the MAPK of the cell
    wall integrity (CWI) pathway. Lack of the nonhomologous region (NHR1-1)
    located centrally in Prs1 abolished the temperature-induced increase in Rlm1
    expression. Furthermore, in vitro point mutations generated in PRS1 corresponding
    to missense mutations associated with human neuropathies or in the
    divalent cation and/or 5-phosphoribosyl-1(a)-pyrophosphate binding sites also
    display increased Rlm1 expression at 30 °C and 37 °C and most give rise to
    caffeine sensitivity. Human PRPS1 cDNA cannot rescue the synthetic lethality
    of a prs1D prs5D strain because it lacks sequences corresponding to NHR1-1 of
    yeast Prs1. The correlation between caffeine sensitivity and increased basal
    expression of Rlm1 in the altered versions of PRS1 can be extended to their
    inability to rescue the synthetic lethality of a prs1D prs5D strain implying that
    impaired CWI may contribute to the observed loss of viability.
    Original languageEnglish
    Pages (from-to)291-301
    Number of pages11
    JournalFEMS Yeast Research
    Volume13
    Issue number3
    Early online date13 Mar 2013
    DOIs
    Publication statusPublished - 8 Apr 2013

    Fingerprint

    Cell Wall
    Cell Survival
    Genes
    Cells
    Maintenance
    Temperature
    Missense Mutation
    Caffeine
    Point Mutation
    Cations
    Complementary DNA
    Binding Sites
    diphosphoric acid
    Synthetic Lethal Mutations
    In Vitro Techniques

    Keywords

    • cell wall integrity, PRPP, synthetic lethality, nonhomologous sequence

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Ugbogu, E. A., Wippler, S. U., Euston, M., Kouwenhoven, E. N., de Brouwer , A. P. M., Schweizer, L. M., & Schweizer, M. (2013). The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability. FEMS Yeast Research, 13(3), 291-301. https://doi.org/10.1111/1567-1364.12033
    Ugbogu, Eziuche Amadike ; Wippler, Sonja Ursula ; Euston, Matthew ; Kouwenhoven, Evelyn N ; de Brouwer , Arjan P M ; Schweizer, Lilian Mary ; Schweizer, Michael. / The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability. In: FEMS Yeast Research. 2013 ; Vol. 13, No. 3. pp. 291-301.
    @article{adb1908a7ab448028b7999980df19d0e,
    title = "The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability",
    abstract = "The gene products of the five-membered PRS gene family in Saccharomyces cerevisiae have been shown to exist as three minimal functional entities, Prs1/ Prs3, Prs2/Prs5, and Prs4/Prs5, each capable of supporting cell viability. The Prs1/Prs3 heterodimer can be regarded as the most important because its loss causes temperature sensitivity. It has been shown that the GFP signal generated by an integrated GFP-Prs1 construct is lost in the absence of Prs3. In addition to interacting with Prs3, Prs1 also interacts with Slt2, the MAPK of the cell wall integrity (CWI) pathway. Lack of the nonhomologous region (NHR1-1) located centrally in Prs1 abolished the temperature-induced increase in Rlm1 expression. Furthermore, in vitro point mutations generated in PRS1 corresponding to missense mutations associated with human neuropathies or in the divalent cation and/or 5-phosphoribosyl-1(a)-pyrophosphate binding sites also display increased Rlm1 expression at 30 °C and 37 °C and most give rise to caffeine sensitivity. Human PRPS1 cDNA cannot rescue the synthetic lethality of a prs1D prs5D strain because it lacks sequences corresponding to NHR1-1 of yeast Prs1. The correlation between caffeine sensitivity and increased basal expression of Rlm1 in the altered versions of PRS1 can be extended to their inability to rescue the synthetic lethality of a prs1D prs5D strain implying that impaired CWI may contribute to the observed loss of viability.",
    keywords = "cell wall integrity, PRPP, synthetic lethality, nonhomologous sequence",
    author = "Ugbogu, {Eziuche Amadike} and Wippler, {Sonja Ursula} and Matthew Euston and Kouwenhoven, {Evelyn N} and {de Brouwer}, {Arjan P M} and Schweizer, {Lilian Mary} and Michael Schweizer",
    note = "{"}NHR1-1 of PRS1 - a link between cell wall integrity and yeast metabolism{"} Subtitle not as the published article - removed.",
    year = "2013",
    month = "4",
    day = "8",
    doi = "10.1111/1567-1364.12033",
    language = "English",
    volume = "13",
    pages = "291--301",
    journal = "FEMS Yeast Research",
    issn = "1567-1356",
    publisher = "Wiley-Blackwell",
    number = "3",

    }

    Ugbogu, EA, Wippler, SU, Euston, M, Kouwenhoven, EN, de Brouwer , APM, Schweizer, LM & Schweizer, M 2013, 'The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability', FEMS Yeast Research, vol. 13, no. 3, pp. 291-301. https://doi.org/10.1111/1567-1364.12033

    The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability. / Ugbogu, Eziuche Amadike; Wippler, Sonja Ursula; Euston, Matthew; Kouwenhoven, Evelyn N; de Brouwer , Arjan P M; Schweizer, Lilian Mary; Schweizer, Michael.

    In: FEMS Yeast Research, Vol. 13, No. 3, 08.04.2013, p. 291-301.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability

    AU - Ugbogu, Eziuche Amadike

    AU - Wippler, Sonja Ursula

    AU - Euston, Matthew

    AU - Kouwenhoven, Evelyn N

    AU - de Brouwer , Arjan P M

    AU - Schweizer, Lilian Mary

    AU - Schweizer, Michael

    N1 - "NHR1-1 of PRS1 - a link between cell wall integrity and yeast metabolism" Subtitle not as the published article - removed.

    PY - 2013/4/8

    Y1 - 2013/4/8

    N2 - The gene products of the five-membered PRS gene family in Saccharomyces cerevisiae have been shown to exist as three minimal functional entities, Prs1/ Prs3, Prs2/Prs5, and Prs4/Prs5, each capable of supporting cell viability. The Prs1/Prs3 heterodimer can be regarded as the most important because its loss causes temperature sensitivity. It has been shown that the GFP signal generated by an integrated GFP-Prs1 construct is lost in the absence of Prs3. In addition to interacting with Prs3, Prs1 also interacts with Slt2, the MAPK of the cell wall integrity (CWI) pathway. Lack of the nonhomologous region (NHR1-1) located centrally in Prs1 abolished the temperature-induced increase in Rlm1 expression. Furthermore, in vitro point mutations generated in PRS1 corresponding to missense mutations associated with human neuropathies or in the divalent cation and/or 5-phosphoribosyl-1(a)-pyrophosphate binding sites also display increased Rlm1 expression at 30 °C and 37 °C and most give rise to caffeine sensitivity. Human PRPS1 cDNA cannot rescue the synthetic lethality of a prs1D prs5D strain because it lacks sequences corresponding to NHR1-1 of yeast Prs1. The correlation between caffeine sensitivity and increased basal expression of Rlm1 in the altered versions of PRS1 can be extended to their inability to rescue the synthetic lethality of a prs1D prs5D strain implying that impaired CWI may contribute to the observed loss of viability.

    AB - The gene products of the five-membered PRS gene family in Saccharomyces cerevisiae have been shown to exist as three minimal functional entities, Prs1/ Prs3, Prs2/Prs5, and Prs4/Prs5, each capable of supporting cell viability. The Prs1/Prs3 heterodimer can be regarded as the most important because its loss causes temperature sensitivity. It has been shown that the GFP signal generated by an integrated GFP-Prs1 construct is lost in the absence of Prs3. In addition to interacting with Prs3, Prs1 also interacts with Slt2, the MAPK of the cell wall integrity (CWI) pathway. Lack of the nonhomologous region (NHR1-1) located centrally in Prs1 abolished the temperature-induced increase in Rlm1 expression. Furthermore, in vitro point mutations generated in PRS1 corresponding to missense mutations associated with human neuropathies or in the divalent cation and/or 5-phosphoribosyl-1(a)-pyrophosphate binding sites also display increased Rlm1 expression at 30 °C and 37 °C and most give rise to caffeine sensitivity. Human PRPS1 cDNA cannot rescue the synthetic lethality of a prs1D prs5D strain because it lacks sequences corresponding to NHR1-1 of yeast Prs1. The correlation between caffeine sensitivity and increased basal expression of Rlm1 in the altered versions of PRS1 can be extended to their inability to rescue the synthetic lethality of a prs1D prs5D strain implying that impaired CWI may contribute to the observed loss of viability.

    KW - cell wall integrity, PRPP, synthetic lethality, nonhomologous sequence

    U2 - 10.1111/1567-1364.12033

    DO - 10.1111/1567-1364.12033

    M3 - Article

    VL - 13

    SP - 291

    EP - 301

    JO - FEMS Yeast Research

    JF - FEMS Yeast Research

    SN - 1567-1356

    IS - 3

    ER -

    Ugbogu EA, Wippler SU, Euston M, Kouwenhoven EN, de Brouwer APM, Schweizer LM et al. The contribution of the non-homologous region of Prs1 to the maintenance of cell wall integrity and cell viability. FEMS Yeast Research. 2013 Apr 8;13(3):291-301. https://doi.org/10.1111/1567-1364.12033