The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization

Michael Lawrence Crichton, David Alexander Muller, Alexandra Christina Isobel Depelsenaire, Frances Elizabeth Pearson, Jonathan Wei, Jacob Coffey, Jin Zhang, Germain J P Fernando, Mark Anthony Fernance Kendall

Research output: Contribution to journalArticle

11 Citations (Scopus)
14 Downloads (Pure)

Abstract

Micro-device use for vaccination has grown in the past decade, with the promise of ease-of-use, painless application, stable solid formulations and greater immune response generation. However, the designs of the highly immunogenic devices (e.g. the gene gun, Nanopatch or laser adjuvantation) require significant energy to enter the skin (30-90 mJ). Within this study, we explore a way to more effectively use energy for skin penetration and vaccination. These modifications change the Nanopatch projections from cylindrical/conical shapes with a density of 20,000 per cm2 to flat-shaped protrusions at 8,000 per cm2, whilst maintaining the surface area and volume that is placed within the skin. We show that this design results in more efficient surface crack initiations, allowing the energy to be more efficiently be deployed through the projections into the skin, with a significant overall increase in penetration depth (50%). Furthermore, we measured a significant increase in localized skin cell death (>2 fold), and resultant infiltrate of cells (monocytes and neutrophils). Using a commercial seasonal trivalent human influenza vaccine (Fluvax 2014), our new patch design resulted in an immune response equivalent to intramuscular injection with approximately 1000 fold less dose, while also being a practical device conceptually suited to widespread vaccination.

Original languageEnglish
Article number27217
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 2 Jun 2016

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization'. Together they form a unique fingerprint.

  • Cite this

    Crichton, M. L., Muller, D. A., Depelsenaire, A. C. I., Pearson, F. E., Wei, J., Coffey, J., Zhang, J., Fernando, G. J. P., & Kendall, M. A. F. (2016). The changing shape of vaccination: Improving immune responses through geometrical variations of a microdevice for immunization. Scientific Reports, 6, [27217]. https://doi.org/10.1038/srep27217