The barley transcription factor HvMYB1 is a positive regulator of drought tolerance

Ross D. Alexander, Charlotte Wendelboe-Nelson, Peter C. Morris

Research output: Contribution to journalArticle

Abstract

Transcription factors such as MYB have previously been associated with the plant response to drought. In this work, studies on the function of the barley (Hordeum vulgare L.) transcription factor HvMYB1 show that gene expression is upregulated in wildtype barley roots and leaves under drought and osmotic stress. Transgenic barley plants that overexpress HvMYB1 were found to be more resistant to drought, showing enhanced relative water content and reduced water loss rate and stomatal conductance as compared to control plants. Levels of the osmolyte proline were enhanced as was expression of dehydrin HvDNH6 in the transgenic lines under drought conditions. The levels of the reactive oxygen species H2O2 were enhanced in wildtype roots and leaves by drought, but less so in the HvMYB1 overexpressing lines. Enzyme activity of the low affinity H2O2 degrading enzyme catalase (EC 1.11.1.6) was also lower in droughted HvMYB1 overexpressing lines. Gene expression of the high affinity ROS scavengers ASCORBATE PEROXIDASE and GLUTATHIONE PEROXIDASE was found to be constitutively high in the overexpressing lines, whereas CATALASE gene expression was similar to the control plants. These results suggest a role for HvMYB1 in protecting plants against drought in the vegetative plant by acting as a mediator of abscisic acid action.
Original languageEnglish
Pages (from-to)246-253
Number of pages8
JournalPlant Physiology and Biochemistry
Volume142
Early online date10 Jul 2019
DOIs
Publication statusPublished - Sep 2019

Fingerprint

drought tolerance
transcription factors
barley
drought
gene expression
catalase
peroxidase
genetically modified organisms
osmotic stress
Hordeum vulgare
stomatal conductance
plant response
abscisic acid
proline
leaves
reactive oxygen species
water stress
enzyme activity
water content
enzymes

Keywords

  • ABA
  • Barley
  • Drought
  • MYB
  • Oxidative stress
  • Proline
  • Reactive oxygen species

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

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abstract = "Transcription factors such as MYB have previously been associated with the plant response to drought. In this work, studies on the function of the barley (Hordeum vulgare L.) transcription factor HvMYB1 show that gene expression is upregulated in wildtype barley roots and leaves under drought and osmotic stress. Transgenic barley plants that overexpress HvMYB1 were found to be more resistant to drought, showing enhanced relative water content and reduced water loss rate and stomatal conductance as compared to control plants. Levels of the osmolyte proline were enhanced as was expression of dehydrin HvDNH6 in the transgenic lines under drought conditions. The levels of the reactive oxygen species H2O2 were enhanced in wildtype roots and leaves by drought, but less so in the HvMYB1 overexpressing lines. Enzyme activity of the low affinity H2O2 degrading enzyme catalase (EC 1.11.1.6) was also lower in droughted HvMYB1 overexpressing lines. Gene expression of the high affinity ROS scavengers ASCORBATE PEROXIDASE and GLUTATHIONE PEROXIDASE was found to be constitutively high in the overexpressing lines, whereas CATALASE gene expression was similar to the control plants. These results suggest a role for HvMYB1 in protecting plants against drought in the vegetative plant by acting as a mediator of abscisic acid action.",
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The barley transcription factor HvMYB1 is a positive regulator of drought tolerance. / Alexander, Ross D.; Wendelboe-Nelson, Charlotte; Morris, Peter C.

In: Plant Physiology and Biochemistry, Vol. 142, 09.2019, p. 246-253.

Research output: Contribution to journalArticle

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