The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success

Steven Footitt, Johanna E. Cornah, Itsara Pracharoenwattana, James H. Bryce, Steven M. Smith

    Research output: Contribution to journalArticle

    Abstract

    The enzyme 3-ketoacyl-CoA thiolase (KAT) (EC 2.3.1.16) catalyses a key step in fatty acid ß-oxidation. In Arabidopsis thaliana, expression of the KAT2 gene is known to be required for the efficient mobilization of triacylglycerol during germination and seedling establishment. Here, data from the Arabidopsis kat2-1 mutant are presented, showing that perturbation of ß-oxidation also affects vegetative growth and reproductive success. In the wild type, the KAT2 protein was detected in all organs tested. In the kat2-1 mutant, rosette leaf area and dry weight, but not leaf number, were greatly increased relative to wild type. Global proliferative arrest of flowering was delayed, resulting in increased silique production in kat2-1 plants. However, total silique dry weight was not increased. kat2-1 siliques were smaller and had a reduced seed number caused by increased ovule abortion. In kat2-1 ovules, carbon flow into sugars via gluconeogeneis and respiration were both reduced in comparison to the wild type. In conclusion, these data indicate that a functional ß-oxidation pathway is required to maintain the balance between silique development and the continued initiation of floral meristems. © The Author [2007]. Published by Oxford University Press [on behalf of the Society for Experimental Biology]. All rights reserved.

    Original languageEnglish
    Pages (from-to)2959-2968
    Number of pages10
    JournalJournal of experimental botany
    Volume58
    Issue number11
    DOIs
    Publication statusPublished - Sep 2007

    Fingerprint

    ovules
    Arabidopsis
    oxidation
    flowering
    mutants
    abortion (plants)
    beta oxidation
    cell respiration
    meristems
    vegetative growth
    leaf area
    Arabidopsis thaliana
    triacylglycerols
    germination
    sugars
    Biological Sciences
    gene expression
    seedlings
    carbon
    enzymes

    Keywords

    • Arabidopsis
    • Beta-oxidation
    • Embryogenesis
    • Flowering
    • Lipid mobilization
    • Peroxisome
    • Respiration

    Cite this

    Footitt, Steven ; Cornah, Johanna E. ; Pracharoenwattana, Itsara ; Bryce, James H. ; Smith, Steven M. / The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success. In: Journal of experimental botany. 2007 ; Vol. 58, No. 11. pp. 2959-2968.
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    The Arabidopsis 3-ketoacyl-CoA thiolase-2 (kat2-1) mutant exhibits increased flowering but reduced reproductive success. / Footitt, Steven; Cornah, Johanna E.; Pracharoenwattana, Itsara; Bryce, James H.; Smith, Steven M.

    In: Journal of experimental botany, Vol. 58, No. 11, 09.2007, p. 2959-2968.

    Research output: Contribution to journalArticle

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