Technique for analysis of bryozoan mitochondrial DNA

J S Porter; D O F Skibinski; J Leamon; P J Hayward

doi:10.1046/j.1471-8278.2000.00018.x

Technique for analysis of bryozoan mitochondrial DNA

J S Porter
, D O F Skibinski
, J Leamon
, P J Hayward

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The use of non-specific mitochondrial DNA (mtDNA) primers can frequently result in the erroneous amplification of DNA from contaminating organisms or from mtDNA nuclear inserts. A reliable method for deriving species-specific mtDNA polymerase chain reaction (PCR) primers using genomic and mtDNA enriched preparations from the same individual is described.

Original language	English
Pages (from-to)	103-105
Number of pages	3
Journal	Molecular Ecology Notes
Volume	1
Issue number	1-2
DOIs	https://doi.org/10.1046/j.1471-8278.2000.00018.x
Publication status	Published - Mar 2001

Keywords

mitochondria
sequences
Bryozoa
nuclear genomes
Alcyonidium diaphanum
mtDNA
PCR

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10.1046/j.1471-8278.2000.00018.x

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title = "Technique for analysis of bryozoan mitochondrial DNA",

abstract = "The use of non-specific mitochondrial DNA (mtDNA) primers can frequently result in the erroneous amplification of DNA from contaminating organisms or from mtDNA nuclear inserts. A reliable method for deriving species-specific mtDNA polymerase chain reaction (PCR) primers using genomic and mtDNA enriched preparations from the same individual is described.",

keywords = "mitochondria, sequences, Bryozoa, nuclear genomes, Alcyonidium diaphanum, mtDNA, PCR",

author = "Porter, \{J S\} and Skibinski, \{D O F\} and J Leamon and Hayward, \{P J\}",

year = "2001",

month = mar,

doi = "10.1046/j.1471-8278.2000.00018.x",

language = "English",

volume = "1",

pages = "103--105",

journal = "Molecular Ecology Notes",

issn = "1471-8278",

publisher = "Wiley-Blackwell Publishing Ltd",

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TY - JOUR

T1 - Technique for analysis of bryozoan mitochondrial DNA

AU - Porter, J S

AU - Skibinski, D O F

AU - Leamon, J

AU - Hayward, P J

PY - 2001/3

Y1 - 2001/3

N2 - The use of non-specific mitochondrial DNA (mtDNA) primers can frequently result in the erroneous amplification of DNA from contaminating organisms or from mtDNA nuclear inserts. A reliable method for deriving species-specific mtDNA polymerase chain reaction (PCR) primers using genomic and mtDNA enriched preparations from the same individual is described.

AB - The use of non-specific mitochondrial DNA (mtDNA) primers can frequently result in the erroneous amplification of DNA from contaminating organisms or from mtDNA nuclear inserts. A reliable method for deriving species-specific mtDNA polymerase chain reaction (PCR) primers using genomic and mtDNA enriched preparations from the same individual is described.

KW - mitochondria

KW - sequences

KW - Bryozoa

KW - nuclear genomes

KW - Alcyonidium diaphanum

KW - mtDNA

KW - PCR

U2 - 10.1046/j.1471-8278.2000.00018.x

DO - 10.1046/j.1471-8278.2000.00018.x

M3 - Article

SN - 1471-8278

VL - 1

SP - 103

EP - 105

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 1-2

ER -

Technique for analysis of bryozoan mitochondrial DNA

Abstract

Keywords

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