Technique for analysis of bryozoan mitochondrial DNA

J S Porter, D O F Skibinski, J Leamon, P J Hayward

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5 Citations (Scopus)

Abstract

The use of non-specific mitochondrial DNA (mtDNA) primers can frequently result in the erroneous amplification of DNA from contaminating organisms or from mtDNA nuclear inserts. A reliable method for deriving species-specific mtDNA polymerase chain reaction (PCR) primers using genomic and mtDNA enriched preparations from the same individual is described.

Original languageEnglish
Pages (from-to)103-105
Number of pages3
JournalMolecular Ecology Notes
Volume1
Issue number1-2
DOIs
Publication statusPublished - Mar 2001

Keywords

  • mitochondria
  • sequences
  • Bryozoa
  • nuclear genomes
  • Alcyonidium diaphanum
  • mtDNA
  • PCR

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