Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis

Oleg N. Reva, Christina Dixelius, Johan Meijer, Fergus G. Priest

    Research output: Contribution to journalArticle

    Abstract

    The phylogenetic relationships of 17 Bacillus strains isolated from plants and soil were determined from partial sequences of genes encoding 16S rRNA, gyraseA (gyrA) and the cheA histidine kinase. Five strains were closely related to Bacillus subtilis subsp. subtilis, three strains were more closely related to B. subtilis subsp. spizizeni and two strains were identified as B. mojavensis. The remaining seven strains formed a cluster closely related to, but distinct from, Bacillus amyloliquefaciens. Some of these strains formed red-pigmented colonies. The abilities of selected strains to survive in the rhizosphere and to colonize plants were studied using oilseed rape (Brassica napus), barley (Hordeum vulgare) and thale cress (Arabidopsis thaliana) as model plants. It was shown by following the titre of bacteria in seedlings and by scanning electron microscopy that survival of Bacillus cells on the roots of seedlings during the first week after treatment of seeds with spore suspensions was crucial for colonization of the rhizosphere and for biocontrol activity. The group of strains related to B. amyloliquefaciens were generally better adapted to colonization of the rhizosphere of plants than other members of the B. subtilis group and could be considered a distinct ecotype of B. amyloliquefaciens. Bacteria in this taxon could be recognized on the basis of amplification of a PCR product with primers directed to the tetB(L) locus but no product with primers directed to the a-amylase gene of B.amyloliquefaciens sensu stricto. © 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)249-259
    Number of pages11
    JournalFEMS Microbiology Ecology
    Volume48
    Issue number2
    DOIs
    Publication statusPublished - 1 May 2004

    Fingerprint

    Bacillus amyloliquefaciens
    colonizing ability
    Bacillus subtilis
    bacteria
    rhizosphere
    Bacillus (bacteria)
    Brassica napus
    Bacillus subtilis subsp. subtilis
    Bacillus mojavensis
    Arabidopsis thaliana
    histidine kinase
    seedlings
    seed treatment
    ecotypes
    amylases
    Hordeum vulgare
    biological control
    scanning electron microscopy
    spores
    barley

    Keywords

    • 16S rRNA
    • Bacillus amyloliquefaciens
    • Bacillus subtilis
    • cheA
    • gyrA
    • Identification
    • Plant colonization

    Cite this

    Reva, Oleg N. ; Dixelius, Christina ; Meijer, Johan ; Priest, Fergus G. / Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis. In: FEMS Microbiology Ecology. 2004 ; Vol. 48, No. 2. pp. 249-259.
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    Taxonomic characterization and plant colonizing abilities of some bacteria related to Bacillus amyloliquefaciens and Bacillus subtilis. / Reva, Oleg N.; Dixelius, Christina; Meijer, Johan; Priest, Fergus G.

    In: FEMS Microbiology Ecology, Vol. 48, No. 2, 01.05.2004, p. 249-259.

    Research output: Contribution to journalArticle

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