Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks

Matthew D. Bourn; Safoura Mohajerani; Georgia Mavria; Nicola Ingram; P. Louise Coletta; Stephen D. Evans; Sally A. Peyman

Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks

Matthew D. Bourn, Safoura Mohajerani, Georgia Mavria, Nicola Ingram, P. Louise Coletta, Stephen D. Evans, Sally A. Peyman^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

This paper reports the use of perfusable microfluidic vasculature networks to investigate the use αvβ3 integrin-targeted liposomes as tumour vasculature targeting agents. Endothelial cells co-cultured with fibroblasts were induced to self-assemble into vasculature networks using pro-angiogenic growth factors. Tumour vasculature was mimicked by growing networks in tumour-cell conditioned media (TCM), taken from HCT116 cell cultures. Assessment of αvβ3 expression using immunostaining and flow cytometry observed the upregulation of αvβ3 expression on endothelial cells grown in TCM. Results observed increased liposome accumulation in tumour conditioned networks, suggesting αvβ3 may be a promising target for anti-cancer therapeutics.

Original language	English
Title of host publication	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021
Publisher	Chemical and Biological Microsystems Society
Pages	325-326
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021 - Palm Springs, Virtual, United States Duration: 10 Oct 2021 → 14 Oct 2021 https://www.microtasconferences.org/microtas2021/

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021
Abbreviated title	MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	10/10/21 → 14/10/21
Internet address	https://www.microtasconferences.org/microtas2021/

Keywords

Liposomes
Organ-on-Chip
Targeting
Tumour Vasculature

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Bourn, M. D., Mohajerani, S., Mavria, G., Ingram, N., Coletta, P. L., Evans, S. D., & Peyman, S. A. (2021). Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks. In 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021 (pp. 325-326). Chemical and Biological Microsystems Society.

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title = "Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks",

abstract = "This paper reports the use of perfusable microfluidic vasculature networks to investigate the use αvβ3 integrin-targeted liposomes as tumour vasculature targeting agents. Endothelial cells co-cultured with fibroblasts were induced to self-assemble into vasculature networks using pro-angiogenic growth factors. Tumour vasculature was mimicked by growing networks in tumour-cell conditioned media (TCM), taken from HCT116 cell cultures. Assessment of αvβ3 expression using immunostaining and flow cytometry observed the upregulation of αvβ3 expression on endothelial cells grown in TCM. Results observed increased liposome accumulation in tumour conditioned networks, suggesting αvβ3 may be a promising target for anti-cancer therapeutics.",

keywords = "Liposomes, Organ-on-Chip, Targeting, Tumour Vasculature",

author = "Bourn, {Matthew D.} and Safoura Mohajerani and Georgia Mavria and Nicola Ingram and Coletta, {P. Louise} and Evans, {Stephen D.} and Peyman, {Sally A.}",

note = "Publisher Copyright: {\textcopyright} 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.; 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, MicroTAS 2021 ; Conference date: 10-10-2021 Through 14-10-2021",

year = "2021",

language = "English",

pages = "325--326",

booktitle = "25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021",

publisher = "Chemical and Biological Microsystems Society",

url = "https://www.microtasconferences.org/microtas2021/",

}

Bourn, MD, Mohajerani, S, Mavria, G, Ingram, N, Coletta, PL, Evans, SD & Peyman, SA 2021, Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks. in 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021. Chemical and Biological Microsystems Society, pp. 325-326, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021, Palm Springs, Virtual, California, United States, 10/10/21.

Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks. / Bourn, Matthew D.; Mohajerani, Safoura; Mavria, Georgia et al.
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021. Chemical and Biological Microsystems Society, 2021. p. 325-326.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks

AU - Bourn, Matthew D.

AU - Mohajerani, Safoura

AU - Mavria, Georgia

AU - Ingram, Nicola

AU - Coletta, P. Louise

AU - Evans, Stephen D.

AU - Peyman, Sally A.

PY - 2021

Y1 - 2021

N2 - This paper reports the use of perfusable microfluidic vasculature networks to investigate the use αvβ3 integrin-targeted liposomes as tumour vasculature targeting agents. Endothelial cells co-cultured with fibroblasts were induced to self-assemble into vasculature networks using pro-angiogenic growth factors. Tumour vasculature was mimicked by growing networks in tumour-cell conditioned media (TCM), taken from HCT116 cell cultures. Assessment of αvβ3 expression using immunostaining and flow cytometry observed the upregulation of αvβ3 expression on endothelial cells grown in TCM. Results observed increased liposome accumulation in tumour conditioned networks, suggesting αvβ3 may be a promising target for anti-cancer therapeutics.

AB - This paper reports the use of perfusable microfluidic vasculature networks to investigate the use αvβ3 integrin-targeted liposomes as tumour vasculature targeting agents. Endothelial cells co-cultured with fibroblasts were induced to self-assemble into vasculature networks using pro-angiogenic growth factors. Tumour vasculature was mimicked by growing networks in tumour-cell conditioned media (TCM), taken from HCT116 cell cultures. Assessment of αvβ3 expression using immunostaining and flow cytometry observed the upregulation of αvβ3 expression on endothelial cells grown in TCM. Results observed increased liposome accumulation in tumour conditioned networks, suggesting αvβ3 may be a promising target for anti-cancer therapeutics.

KW - Liposomes

KW - Organ-on-Chip

KW - Targeting

KW - Tumour Vasculature

UR - http://www.scopus.com/inward/record.url?scp=85136983314&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:85136983314

SP - 325

EP - 326

BT - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021

PB - Chemical and Biological Microsystems Society

T2 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2021

Y2 - 10 October 2021 through 14 October 2021

ER -

Targeting Tumour Vasculature using Integrin αvβ3 -Observation of Liposome Accumulation in Microfluidic Vasculature Networks

Abstract

Conference

Keywords

ASJC Scopus subject areas

UN SDGs

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Cite this