Synthesis of stable 2D micro-assemblies of DNA tiles achieved via intrinsic curvatures in the skeleton of DNA duplexes coupled with the flexible support of the twisted side-arms

Mirza Muhammad Faran Ashraf Baig, Xiuli Gao, Awais Farid, Abdul Wasy Zia, Muhammad Abbas, Hongkai Wu

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

The science of DNA nanotechnology has led to the synthesis of size-controllable materials with uniformed geometries at nano- and micro-scales. Herein, we proposed the double-crossover (DX), antiparallel, and even half-turns perimeter tiles (DAE tiles) to synthesize intact, mono-crystalline, and giant 2D DNA micro-assemblies. The DNA tiles with 10-half-turns perimeter were synthesized via self-assembly of 106 nucleotides (NT) circular scaffold along with the complimentary staple strands. The DAE DNA tiles were successfully polymerized to achieve stable lattices by adjusting the inter-tile distances of certain lengths for attaining torsional (or twisting) chirality. We determined that the inter-tile connections affected the degree of coiling (or super-coiling) and twisting forces (right- or left-handed twists) in the DNA helix. While the degree of polymerization of DNA tiles was also tune-able by controlling the lengths and structural designs of the circular core of the DNA tiles. Furthermore, the number of half-turns in the core and on the connection arms (4 or 5) with even “E” or odd “O” half-turns was crucial. It affected the direction of winding of DNA duplexes to alter the overall stiffness and sturdiness of DNA lattices. The number of half-turns in the connections were either “4 or Even (21 NT); E with 5 NT sticky ends” or “5 Odd (26 NT); O with 6 NT sticky ends” (DAE-E or DAE-O tile systems). The AFM results revealed that the above tile systems (DAE-E or DAE-O) together with the locations of crossovers, and holiday junctions along the DNA tiles controlled the left- or right-handed coiling of DNA double strands. This phenomenon affected the compactness of resulting DNA motifs, the overall intrinsic curvatures, double-strand packing, and the geometry of DNA lattices.
Original languageEnglish
Pages (from-to)4279–4289
Number of pages11
JournalApplied Nanoscience
Volume13
Issue number6
Early online date1 Nov 2022
DOIs
Publication statusPublished - Jun 2023

Keywords

  • 2D DNA assemblies
  • Connection arms
  • Double crossover (DX) DNA tiles
  • Mono-crystalline DNA lattices
  • Polymerization
  • Torsional chirality

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electrical and Electronic Engineering
  • Biotechnology
  • Cell Biology
  • Physical and Theoretical Chemistry
  • Materials Science (miscellaneous)

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