Synthesis of α-amylase and α-glucosidase by membrane bound ribosomes from Bacilluslicheniformis

M. Thirunavukkarasu, Fergus G. Priest

    Research output: Contribution to journalArticle

    Abstract

    a-Glucosidase was membrane bound during exponential growth of Bacilluslicheniformis but was released into the medium during stationary phase. It could be partially removed from exponential phase cells by washing with NaCl (0.5 M). a-Amylase was exclusively extracellular and could not be detected in cells. Polysomes were prepared from exponential phase cells and separated into membrane bound and soluble fractions. Invitro chain completion and immunoprecipitation showed that a-glucosidase and a-amylase were synthesized by membrane bound and not by soluble ribosomes. © 1983.

    Original languageEnglish
    Pages (from-to)677-683
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume114
    Issue number2
    Publication statusPublished - 29 Jul 1983

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