Susceptibility of scleroglucan to the (1→3)-β-d-glucanase zymolyase

Brian J. Catley, Margaret E Fraser

    Research output: Contribution to journalArticle

    Abstract

    Exposure of the (1?3,6)-ß-d-glucan, scleroglucan, to 0.2m NaOH at room temperature rendered it susceptible to hydrolysis by the (1?3)-ß-d-glucanase zymolyase. Fractionation of the products revealed that 42% of the polysaccharide remained intact and that the remainder was converted into glucose, laminaribiose, and gentiobiose. The implications of these findings for selectively solubilising glucans in yeast cell walls prior to their enzymic characterisation are discussed. © 1990.

    Original languageEnglish
    Pages (from-to)83-88
    Number of pages6
    JournalCarbohydrate Research
    Volume183
    Issue number1
    Publication statusPublished - 15 Nov 1988

    Fingerprint

    Glucans
    Fractionation
    Yeast
    Polysaccharides
    Hydrolysis
    Cells
    Glucose
    Temperature
    zymolyase
    scleroglucan
    laminaribiose
    gentiobiose

    Cite this

    Catley, Brian J. ; Fraser, Margaret E. / Susceptibility of scleroglucan to the (1→3)-β-d-glucanase zymolyase. In: Carbohydrate Research. 1988 ; Vol. 183, No. 1. pp. 83-88.
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    abstract = "Exposure of the (1?3,6)-{\ss}-d-glucan, scleroglucan, to 0.2m NaOH at room temperature rendered it susceptible to hydrolysis by the (1?3)-{\ss}-d-glucanase zymolyase. Fractionation of the products revealed that 42{\%} of the polysaccharide remained intact and that the remainder was converted into glucose, laminaribiose, and gentiobiose. The implications of these findings for selectively solubilising glucans in yeast cell walls prior to their enzymic characterisation are discussed. {\circledC} 1990.",
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    Catley, BJ & Fraser, ME 1988, 'Susceptibility of scleroglucan to the (1→3)-β-d-glucanase zymolyase', Carbohydrate Research, vol. 183, no. 1, pp. 83-88.

    Susceptibility of scleroglucan to the (1→3)-β-d-glucanase zymolyase. / Catley, Brian J.; Fraser, Margaret E.

    In: Carbohydrate Research, Vol. 183, No. 1, 15.11.1988, p. 83-88.

    Research output: Contribution to journalArticle

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