Survey of restriction-modification systems and transformation in Mannheimia haemolytica and Pasteurella trehalosi

A. E. Hill, F. A. Lainson

    Research output: Contribution to journalArticle

    Abstract

    A significant obstacle to molecular studies of Mannheimia (Pasteurella) haemolytica, has been its resistance to genetic transformation. The lack of competence of many M. haemolytica strains has been attributed to the presence of restriction modification systems. In this study, representative strains of 12 M. haemolytica serotypes and four Pasteurella trehalosi serotypes were successfully transformed by electroporation using a recombinant vector derived from the native M. haemolytica A1 serotype plasmid pNSF2176. Transformation was achieved despite PCR-based evidence for the presence of genes encoding a type I restriction enzyme, phaI, and a type II restriction enzyme hsdM, in each of the M. haemolytica strains. © 2002 Elsevier Science B.V. All rights reserved.

    Original languageEnglish
    Pages (from-to)103-109
    Number of pages7
    JournalVeterinary Microbiology
    Volume92
    Issue number1-2
    DOIs
    Publication statusPublished - 20 Mar 2003

    Fingerprint

    Pasteurella trehalosi
    Mannheimia haemolytica
    restriction endonucleases
    serotypes
    Mannheimia
    electroporation
    genetic transformation
    enzymes
    plasmids

    Keywords

    • Electroporation
    • hsdM
    • Mannheimia haemolytica
    • Pasteurella trehalosi
    • phaI
    • Transformation

    Cite this

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    abstract = "A significant obstacle to molecular studies of Mannheimia (Pasteurella) haemolytica, has been its resistance to genetic transformation. The lack of competence of many M. haemolytica strains has been attributed to the presence of restriction modification systems. In this study, representative strains of 12 M. haemolytica serotypes and four Pasteurella trehalosi serotypes were successfully transformed by electroporation using a recombinant vector derived from the native M. haemolytica A1 serotype plasmid pNSF2176. Transformation was achieved despite PCR-based evidence for the presence of genes encoding a type I restriction enzyme, phaI, and a type II restriction enzyme hsdM, in each of the M. haemolytica strains. {\circledC} 2002 Elsevier Science B.V. All rights reserved.",
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    Survey of restriction-modification systems and transformation in Mannheimia haemolytica and Pasteurella trehalosi. / Hill, A. E.; Lainson, F. A.

    In: Veterinary Microbiology, Vol. 92, No. 1-2, 20.03.2003, p. 103-109.

    Research output: Contribution to journalArticle

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    AU - Lainson, F. A.

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