Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding

Simon R. Corrie; Germain J.P. Fernando; Michael L. Crichton; Marion E.G. Brunck; Chris D. Anderson; Mark A.F. Kendall

doi:10.1039/c0lc00068j

Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding

Simon R. Corrie, Germain J.P. Fernando, Michael L. Crichton, Marion E.G. Brunck, Chris D. Anderson, Mark A.F. Kendall

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

Minimally invasive biosensors are of great interest for rapid detection of disease biomarkers for diagnostic screening at the point-of-care. Here we introduce a device which extracts disease-specific biomarkers directly from the upper dermis, without the needle and syringe or resource-intensive blood processing. Using antigen-specific antibodies raised in mice as a model system, we confirm the analytical specificity and sensitivity of the antibody capture and extraction in comparison to the conventional methods based on needle/syringe blood draw followed by processing and antigen-specific ELISAs.

Original language	English
Pages (from-to)	2655-2658
Number of pages	4
Journal	Lab on a Chip
Volume	10
Issue number	20
DOIs	https://doi.org/10.1039/c0lc00068j
Publication status	Published - 21 Oct 2010

ASJC Scopus subject areas

Bioengineering
General Chemistry
Biochemistry
Biomedical Engineering

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10.1039/c0lc00068j

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AU - Brunck, Marion E.G.

AU - Anderson, Chris D.

AU - Kendall, Mark A.F.

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AB - Minimally invasive biosensors are of great interest for rapid detection of disease biomarkers for diagnostic screening at the point-of-care. Here we introduce a device which extracts disease-specific biomarkers directly from the upper dermis, without the needle and syringe or resource-intensive blood processing. Using antigen-specific antibodies raised in mice as a model system, we confirm the analytical specificity and sensitivity of the antibody capture and extraction in comparison to the conventional methods based on needle/syringe blood draw followed by processing and antigen-specific ELISAs.

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Surface-modified microprojection arrays for intradermal biomarker capture, with low non-specific protein binding

Abstract

ASJC Scopus subject areas

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