Surface attachment induced production of antimicrobial compounds by marine epiphytic bacteria using modified roller bottle cultivation

Liming Yan, Kenneth G. Boyd, J. Grant Burgess

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    A modified roller bottle culture method elicited the production of antimicrobial compounds from 2 epibiotic marine bacterial strains, EI-34-6 and II-111-5, isolated from the surface of the marine alga Palmaria palmata. These isolates, tentatively identified as Bacillus species, were grown as a biofilm on the surface of nutrient glycerol ferric agar (NGFA) and marine Columbia glycerol agar (MCGA) on the inside of a rolling bottle. The biofilm was shown to be stable, and the cells were difficult to remove from the agar surface. The culture supernatant exhibited a different antibiotic spectrum when the strains were grown using the agar roller bottle method compared with shake flask cultures or nonagar roller bottle cultures. These results suggest that biofilm formation is an important factor in the production of antimicrobial compounds by these 2 strains, and roller bottle cultivation also allowed production of these compounds to be increased. The methodology used here has the potential to allow increased production of useful secondary metabolites such as antibiotics from marine epibiotic bacteria.

    Original languageEnglish
    Pages (from-to)356-366
    Number of pages11
    JournalMarine Biotechnology
    Volume4
    Issue number4
    DOIs
    Publication statusPublished - Jul 2002

    Keywords

    • Antimicrobial compounds
    • Bioreactor
    • Marine epiphytic bacteria
    • Roller bottle cultivation
    • Surface attachment

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