TY - JOUR
T1 - Super-Resolution Fluorescence Microscopy Methods for Assessing Mouse Biology
AU - Valli, Jessica
AU - Sanderson, Jeremy
N1 - Funding Information:
J.V. is supported by the Wellcome Trust, grant 208345/Z/17/Z. J.S. is supported by the Medical Research Council. Imaging was performed at the Edinburgh Super Resolution Imaging Consortium (ESRIC) at Heriot-Watt Univeristy, the Bioimaging Unit at the MRC Harwell Institute's Mammalian Genetics Unit, and at the Octopus Imaging Cluster at the Central Laser Facility.
Funding Information:
J.V. is supported by the Wellcome Trust, grant 208345/Z/17/Z. J.S. is supported by the Medical Research Council. Imaging was performed at the Edinburgh Super Resolution Imaging Consortium (ESRIC) at Heriot‐Watt Univeristy, the Bioimaging Unit at the MRC Harwell Institute's Mammalian Genetics Unit, and at the Octopus Imaging Cluster at the Central Laser Facility.
Publisher Copyright:
© 2021 The Authors. Current Protocols published by Wiley Periodicals LLC.
PY - 2021/8/26
Y1 - 2021/8/26
N2 - Super-resolution (diffraction unlimited) microscopy was developed 15 years ago; the developers were awarded the Nobel Prize in Chemistry in recognition of their work in 2014. Super-resolution microscopy is increasingly being applied to diverse scientific fields, from single molecules to cell organelles, viruses, bacteria, plants, and animals, especially the mammalian model organism Mus musculus. In this review, we explain how super-resolution microscopy, along with fluorescence microscopy from which it grew, has aided the renaissance of the light microscope. We cover experiment planning and specimen preparation and explain structured illumination microscopy, super-resolution radial fluctuations, stimulated emission depletion microscopy, single-molecule localization microscopy, and super-resolution imaging by pixel reassignment. The final section of this review discusses the strengths and weaknesses of each super-resolution technique and how to choose the best approach for your research.
AB - Super-resolution (diffraction unlimited) microscopy was developed 15 years ago; the developers were awarded the Nobel Prize in Chemistry in recognition of their work in 2014. Super-resolution microscopy is increasingly being applied to diverse scientific fields, from single molecules to cell organelles, viruses, bacteria, plants, and animals, especially the mammalian model organism Mus musculus. In this review, we explain how super-resolution microscopy, along with fluorescence microscopy from which it grew, has aided the renaissance of the light microscope. We cover experiment planning and specimen preparation and explain structured illumination microscopy, super-resolution radial fluctuations, stimulated emission depletion microscopy, single-molecule localization microscopy, and super-resolution imaging by pixel reassignment. The final section of this review discusses the strengths and weaknesses of each super-resolution technique and how to choose the best approach for your research.
KW - pixel reassignment
KW - single molecule localization microscopy
KW - stimulated emission depletion microscopy
KW - structured illumination microscopy
KW - super resolution microscopy
KW - super resolution radial fluctuations
UR - http://www.scopus.com/inward/record.url?scp=85113606006&partnerID=8YFLogxK
U2 - 10.1002/cpz1.224
DO - 10.1002/cpz1.224
M3 - Article
C2 - 34436832
AN - SCOPUS:85113606006
SN - 2691-1299
VL - 1
JO - Current Protocols
JF - Current Protocols
IS - 8
M1 - e224
ER -