TY - JOUR
T1 - Sulfoevernan, a polyanionic polysaccharide, and the narcissus lectin potently inhibit HIV infection by binding to viral envelope protein
AU - Weiler, B.E.
AU - Schröder, H.C.
AU - Stefanovich, V.
AU - Stewart, D.
AU - Forrest, J.M.S.
AU - Allen, L.B.
AU - Bowden, B.J.
AU - Kreuter, M.H.
AU - Voth, R.
AU - Müller, W.E.G.
PY - 1990/9
Y1 - 1990/9
N2 - Sulphoevernan is a sulphated a-1 ? 3, 1 ? 4 polyglucan (Mr 20000) with a helical structure. This compound effectively inhibits both human immunodeficiency virus type 1 (HIV-1) and type 2 infection of cells in vitro at concentrations around 0.5 µg/ml. Moreover, the compound completely inhibits HIV-1-induced syncytium formation at a concentration of 1 µg/ml. Competition experiments with 35S-labelled sulphoevernan revealed that the mannose-specific lectin from Narcissus pseudonarcissus prevented binding of sulphoevernan to HIV-1, whereas the antibody OKT4A did not reduce the amount of sulphoevernan bound to MT-2 cells. These data indicate that the non-cytotoxic polymer sulphoevernan binds to the virus rather than to the host cell. In vivo studies, using Rauscher leukaemia virus in NMRI mice, revealed that, at a daily dose of 20 mg/kg, the animals were protected against virus-induced increases in spleen weight. From these in vitro and in vivo data we conclude that sulphoevernan has potential in the treatment of acquired immunodeficiency syndrome.© SOCIETY FOR GENERAL MICROBIOLOGY
AB - Sulphoevernan is a sulphated a-1 ? 3, 1 ? 4 polyglucan (Mr 20000) with a helical structure. This compound effectively inhibits both human immunodeficiency virus type 1 (HIV-1) and type 2 infection of cells in vitro at concentrations around 0.5 µg/ml. Moreover, the compound completely inhibits HIV-1-induced syncytium formation at a concentration of 1 µg/ml. Competition experiments with 35S-labelled sulphoevernan revealed that the mannose-specific lectin from Narcissus pseudonarcissus prevented binding of sulphoevernan to HIV-1, whereas the antibody OKT4A did not reduce the amount of sulphoevernan bound to MT-2 cells. These data indicate that the non-cytotoxic polymer sulphoevernan binds to the virus rather than to the host cell. In vivo studies, using Rauscher leukaemia virus in NMRI mice, revealed that, at a daily dose of 20 mg/kg, the animals were protected against virus-induced increases in spleen weight. From these in vitro and in vivo data we conclude that sulphoevernan has potential in the treatment of acquired immunodeficiency syndrome.© SOCIETY FOR GENERAL MICROBIOLOGY
U2 - 10.1099/0022-1317-71-9-1957
DO - 10.1099/0022-1317-71-9-1957
M3 - Article
SN - 1465-2099
VL - 71
SP - 1957
EP - 1963
JO - Journal of General Virology
JF - Journal of General Virology
ER -