Subcellular components of Vibrio harveyi and probiotics induce immune responses in rainbow trout, Oncorhynchus mykiss (Walbaum), against V. harveyi

S. Arijo, J. Brunt, M. Chabrillón, P. Díaz-Rosales, B. Austin

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    Bacterial subcellular components and probiotics were successful for the stimulation of immunity and the prevention of Vibrio harveyi infections in rainbow trout, Oncorhynchus mykiss (Walbaum). Rainbow trout were immunized with whole inactivated cells of V. harveyi to obtain polyclonal antibodies against specific antigens. Western blotting showed a unique reactive band (~93 kDa) between serum and bacterial proteins from outer membrane proteins (OMP) and extracellular products (ECP). Probiotics were selected according to their capability to inhibit V. harveyi. Two of these bacteria, i.e. A3-47 and A3-51, showed cross-reactivity with V. harveyi antiserum. Their OMPs and ECPs were reactive with V. harveyi antiserum in bands of ~93 kDa for A3-51 and higher for A3-47. In vivo tests determined that fish fed with A3-51 produced cross-reactive antibodies against V. harveyi and also, the survival of these fish infected with V. harveyi was high, being similar to the level achieved with vaccinated fish. Thus, the probiotics, when administered as live preparations, were capable of producing cross-reactive antibody against specific bacterial pathogens. © 2008 The Authors.

    Original languageEnglish
    Pages (from-to)579-590
    Number of pages12
    JournalJournal of Fish Diseases
    Volume31
    Issue number8
    DOIs
    Publication statusPublished - Aug 2008

    Keywords

    • Extracellular product
    • Outer membrane protein
    • Probiotic
    • Rainbow trout
    • Vaccine
    • Vibrio harveyi

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