Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish

C. S. Ramsden; T. B. Henry; R. D. Handy

doi:10.1016/j.aquatox.2012.08.021

Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish

C. S. Ramsden, T. B. Henry, R. D. Handy^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

There are limited data on the sub-lethal physiological effects of titanium dioxide nanoparticles (TiO2 NPs) in adult fishes, and the consequences of TiO2 NP exposure on reproductive success are also unclear. This study aimed to examine the sub-lethal effects of a 14-d aqueous TiO2 (TiO2 NP, 0.1 or 1.0 mgl(-1); TiO2 bulk, 1.0 mgl(-1)) exposure on the physiology and reproductive health of zebrafish. After the 14-d exposure, fish were examined for haematology, whole body electrolyte and trace metal profiles, biochemistry, and histopathology. Then, during a 21-d post exposure recovery period, effects of the TiO2 exposure on reproductive success were evaluated. Whole body Ti concentrations increased significantly in fish exposed to both the 1.0 mgl(-1) TiO2 NP and bulk TiO2 compared to controls, but concentrations returned to control levels by the end of the recovery period. No change in erythrocyte counts were observed, but there was a two-fold decline in leukocyte counts in all TiO2 treatment groups relative to time-matched controls. Whole body electrolyte and trace metal profiles were not affected by exposure to TiO2, and there were no changes in Na+K+-ATPase activity in brain, gill or liver tissues. Total glutathione (GSH) levels in brain, gill and liver tissues were higher in fish exposed to TiO2 NP (both 0.1 and 1.0 mgl(-1)) compared to bulk TiO2 and control fish. Histological examination of gill, liver, brain and gonad tissues showed little evidence of treatment-related morphological change. At the end of the 14-d exposure adult zebrafish were able to reproduce; however, the cumulative number of viable embryos produced was lower in fish exposed to 1.0 mgl(-1) TiO2 (both NP and bulk) by the end of the 21-d recovery period. Overall, this study showed limited toxicity of bulk or nano scale TiO2 during the exposure; however reproduction was affected in both bulk and NP 1.0 mgl(-1) groups. (C) 2012 Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	404-413
Number of pages	10
Journal	Aquatic Toxicology
Volume	126
DOIs	https://doi.org/10.1016/j.aquatox.2012.08.021
Publication status	Published - 15 Jan 2013

Keywords

Electrolytes
Glutathione
CARBON NANOTUBES
ECOTOXICOLOGY
RAINBOW-TROUT
ENVIRONMENTAL-IMPACT
Danio rerio
Nanomaterials
DIETARY EXPOSURE
Fecundity
Oxidative stress
TROUT ONCORHYNCHUS-MYKISS
MANUFACTURED NANOMATERIALS
OXIDATIVE STRESS
DANIO-RERIO
TOXICITY

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10.1016/j.aquatox.2012.08.021

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@article{2f488d20a67c4db08fc36455e6719c52,

title = "Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish",

abstract = "There are limited data on the sub-lethal physiological effects of titanium dioxide nanoparticles (TiO2 NPs) in adult fishes, and the consequences of TiO2 NP exposure on reproductive success are also unclear. This study aimed to examine the sub-lethal effects of a 14-d aqueous TiO2 (TiO2 NP, 0.1 or 1.0 mgl(-1); TiO2 bulk, 1.0 mgl(-1)) exposure on the physiology and reproductive health of zebrafish. After the 14-d exposure, fish were examined for haematology, whole body electrolyte and trace metal profiles, biochemistry, and histopathology. Then, during a 21-d post exposure recovery period, effects of the TiO2 exposure on reproductive success were evaluated. Whole body Ti concentrations increased significantly in fish exposed to both the 1.0 mgl(-1) TiO2 NP and bulk TiO2 compared to controls, but concentrations returned to control levels by the end of the recovery period. No change in erythrocyte counts were observed, but there was a two-fold decline in leukocyte counts in all TiO2 treatment groups relative to time-matched controls. Whole body electrolyte and trace metal profiles were not affected by exposure to TiO2, and there were no changes in Na+K+-ATPase activity in brain, gill or liver tissues. Total glutathione (GSH) levels in brain, gill and liver tissues were higher in fish exposed to TiO2 NP (both 0.1 and 1.0 mgl(-1)) compared to bulk TiO2 and control fish. Histological examination of gill, liver, brain and gonad tissues showed little evidence of treatment-related morphological change. At the end of the 14-d exposure adult zebrafish were able to reproduce; however, the cumulative number of viable embryos produced was lower in fish exposed to 1.0 mgl(-1) TiO2 (both NP and bulk) by the end of the 21-d recovery period. Overall, this study showed limited toxicity of bulk or nano scale TiO2 during the exposure; however reproduction was affected in both bulk and NP 1.0 mgl(-1) groups. (C) 2012 Elsevier B.V. All rights reserved.",

keywords = "Electrolytes, Glutathione, CARBON NANOTUBES, ECOTOXICOLOGY, RAINBOW-TROUT, ENVIRONMENTAL-IMPACT, Danio rerio, Nanomaterials, DIETARY EXPOSURE, Fecundity, Oxidative stress, TROUT ONCORHYNCHUS-MYKISS, MANUFACTURED NANOMATERIALS, OXIDATIVE STRESS, DANIO-RERIO, TOXICITY",

author = "Ramsden, {C. S.} and Henry, {T. B.} and Handy, {R. D.}",

year = "2013",

month = jan,

day = "15",

doi = "10.1016/j.aquatox.2012.08.021",

language = "English",

volume = "126",

pages = "404--413",

journal = "Aquatic Toxicology",

issn = "0166-445X",

publisher = "Elsevier",

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TY - JOUR

T1 - Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish

AU - Ramsden, C. S.

AU - Henry, T. B.

AU - Handy, R. D.

PY - 2013/1/15

Y1 - 2013/1/15

N2 - There are limited data on the sub-lethal physiological effects of titanium dioxide nanoparticles (TiO2 NPs) in adult fishes, and the consequences of TiO2 NP exposure on reproductive success are also unclear. This study aimed to examine the sub-lethal effects of a 14-d aqueous TiO2 (TiO2 NP, 0.1 or 1.0 mgl(-1); TiO2 bulk, 1.0 mgl(-1)) exposure on the physiology and reproductive health of zebrafish. After the 14-d exposure, fish were examined for haematology, whole body electrolyte and trace metal profiles, biochemistry, and histopathology. Then, during a 21-d post exposure recovery period, effects of the TiO2 exposure on reproductive success were evaluated. Whole body Ti concentrations increased significantly in fish exposed to both the 1.0 mgl(-1) TiO2 NP and bulk TiO2 compared to controls, but concentrations returned to control levels by the end of the recovery period. No change in erythrocyte counts were observed, but there was a two-fold decline in leukocyte counts in all TiO2 treatment groups relative to time-matched controls. Whole body electrolyte and trace metal profiles were not affected by exposure to TiO2, and there were no changes in Na+K+-ATPase activity in brain, gill or liver tissues. Total glutathione (GSH) levels in brain, gill and liver tissues were higher in fish exposed to TiO2 NP (both 0.1 and 1.0 mgl(-1)) compared to bulk TiO2 and control fish. Histological examination of gill, liver, brain and gonad tissues showed little evidence of treatment-related morphological change. At the end of the 14-d exposure adult zebrafish were able to reproduce; however, the cumulative number of viable embryos produced was lower in fish exposed to 1.0 mgl(-1) TiO2 (both NP and bulk) by the end of the 21-d recovery period. Overall, this study showed limited toxicity of bulk or nano scale TiO2 during the exposure; however reproduction was affected in both bulk and NP 1.0 mgl(-1) groups. (C) 2012 Elsevier B.V. All rights reserved.

AB - There are limited data on the sub-lethal physiological effects of titanium dioxide nanoparticles (TiO2 NPs) in adult fishes, and the consequences of TiO2 NP exposure on reproductive success are also unclear. This study aimed to examine the sub-lethal effects of a 14-d aqueous TiO2 (TiO2 NP, 0.1 or 1.0 mgl(-1); TiO2 bulk, 1.0 mgl(-1)) exposure on the physiology and reproductive health of zebrafish. After the 14-d exposure, fish were examined for haematology, whole body electrolyte and trace metal profiles, biochemistry, and histopathology. Then, during a 21-d post exposure recovery period, effects of the TiO2 exposure on reproductive success were evaluated. Whole body Ti concentrations increased significantly in fish exposed to both the 1.0 mgl(-1) TiO2 NP and bulk TiO2 compared to controls, but concentrations returned to control levels by the end of the recovery period. No change in erythrocyte counts were observed, but there was a two-fold decline in leukocyte counts in all TiO2 treatment groups relative to time-matched controls. Whole body electrolyte and trace metal profiles were not affected by exposure to TiO2, and there were no changes in Na+K+-ATPase activity in brain, gill or liver tissues. Total glutathione (GSH) levels in brain, gill and liver tissues were higher in fish exposed to TiO2 NP (both 0.1 and 1.0 mgl(-1)) compared to bulk TiO2 and control fish. Histological examination of gill, liver, brain and gonad tissues showed little evidence of treatment-related morphological change. At the end of the 14-d exposure adult zebrafish were able to reproduce; however, the cumulative number of viable embryos produced was lower in fish exposed to 1.0 mgl(-1) TiO2 (both NP and bulk) by the end of the 21-d recovery period. Overall, this study showed limited toxicity of bulk or nano scale TiO2 during the exposure; however reproduction was affected in both bulk and NP 1.0 mgl(-1) groups. (C) 2012 Elsevier B.V. All rights reserved.

KW - Electrolytes

KW - Glutathione

KW - CARBON NANOTUBES

KW - ECOTOXICOLOGY

KW - RAINBOW-TROUT

KW - ENVIRONMENTAL-IMPACT

KW - Danio rerio

KW - Nanomaterials

KW - DIETARY EXPOSURE

KW - Fecundity

KW - Oxidative stress

KW - TROUT ONCORHYNCHUS-MYKISS

KW - MANUFACTURED NANOMATERIALS

KW - OXIDATIVE STRESS

KW - DANIO-RERIO

KW - TOXICITY

U2 - 10.1016/j.aquatox.2012.08.021

DO - 10.1016/j.aquatox.2012.08.021

M3 - Article

C2 - 23084046

SN - 0166-445X

VL - 126

SP - 404

EP - 413

JO - Aquatic Toxicology

JF - Aquatic Toxicology

ER -

Sub-lethal effects of titanium dioxide nanoparticles on the physiology and reproduction of zebrafish

Abstract

Keywords

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