Studies on carbohydrate-metabolising enzymes. Part XX. Sweet-corn debranching enzymes

David J Manners, Karen L Rowe

Research output: Contribution to journalArticle

Abstract

Extracts of sweet corn contain a mixture of debranching enzymes which have been separated to give a typical R-enzyme (which acts only on amylopectin) and an isoamylase (which acts on both glycogen and amylopectin). The action of isoamylase on phytoglycogen gives a mixture of maltosaccharides and a residual polysaccharide which had a glycogen rather than an amylopectin-type structure. This suggests that, in vivo, amylopectin is not formed by the enzymic debranching of a glycogen precursor. Sweet-corn extracts also contain a third a-(1?6)-glucosidase which hydrolyses pullulan. © 1969.

Original languageEnglish
Pages (from-to)107-121
Number of pages15
JournalCarbohydrate Research
Volume9
Issue number1
Publication statusPublished - Jan 1969

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